Nonlinear Magnetochemical Effects in Nanotherapy of Walker-256 Carcinosarcoma.

The biological and medical aspects of magnetochemical effects in nanotherapy of tumors remain poorly studied. The present paper investigates the influence of nonlinear magnetochemical effects of anisotropic magnetic nanodots on an animal tumor model. The magnetic properties and electron spin resonance spectra of magnetic nanodots and doxorubicin were investigated after mechano-magnetochemical synthesis.

Efficacy of Combined Regional Inductive Moderate Hyperthermia and Chemotherapy in Patients With Multiple Liver Metastases From Breast Cancer.

Purpose: Regional inductive moderate hyperthermia in combination with chemotherapy can improve the therapeutic efficacy in patients with breast cancer with multiple liver metastases.

Methods: The study included 103 patients with breast cancer with multiple liver metastases: 53 patients (main group) who received a combined chemotherapy (TC drug combination) and regional inductive moderate hyperthermia treatment and 50 patients (control group) who received chemotherapy (TC drug combination) alone. Regional inductive moderate hyperthermia exploited electromagnetic fields with an operating frequency of 27.

[Species-specific localization of DNA from pericentromeric heterochromatin on polytene chromosomes in the salivary gland cells and 3D-nuclear organization nurse cells in Drosophila virilis, and Drosophila kanekoi (Diptera: Drosophilidae)].

Microdissection of the chromocenter of D. virilis salivary gland polytene chromosomes has been carried out and the region-specific. DNA library (DvirIII) has been obtained.

[Comparison of nucleotide sequences of ribosomal DNA of the mosquito genera Aedes and Ochlerotatus (Diptera: Culicidae: Aedini)].

Morphological study allowed identifying 9 species of mosquitoes of the genus Ochlerotatus and 2 species of the genus Aedes. Sequencing of the rDNA region was performed for all these speciemens. The sequences of rDNA were the following: A.

[Molecular genetic analysis of the X-chromosomal nuclear envelope attachment region in nurse cells of the malaria mosquitoes Anopheles messeae Fall].

DNA of the X-chromosomal nuclear envelope attachment region was isolated from malaria mosquito Anopheles messeae Fall. nurse cells by chromosome microdissection. A DNA library of the region was constructed using a plasmid vector.

[Study of the organization of polytene chromosomes in the nuclei of Drosophila melanogaster pseudonurse cells with the use of the DNA library of the Drosophila orena chromocenter].

The location of the Drosophila orena chromocenter in polytene chromosomes of pseudonurse cells of the D. melanogaster ovaries (the otu11 mutation) and salivary glands has been studied. Numerous sites of location of the D.

[Analysis of the chromocenter DNA composition in polytene chromosomes of Drosophila orena ovarian nurse cells].

Chromocenter DNA fragments of polytene chromosomes of Drosophila orena ovarian nurse cells were cloned from a region-specific library (Dore 1) in a plasmid vector to yield 133 clones. A total of 76 clones were selected and sequenced. The total length of the sequenced fragments was 23940 bp.

[Inversion polymorphism in malarial mosquito Anopheles messeae natural populations from the north-eastern region of the range].

A cytogenetic study of eight natural populations of Anopheles messeae from the north-eastern part of the species areal was conducted. Complete predominance of homozygotes XL11 and 3R11 in the northern populations was observed. Change in the chromosome 2 inversion frequencies from south northwards was shown.

[Molecular composition of the chromosome 2 pericentric heterochromatin in malarial mosquitoes (Diptera, Culicidae)].

Rich in repeated DNA sequences and poor in genes, the heterochromatin is an important functional part of the eukaryotic genome. Heterochromatin exhibits high evolutionary variability, which was revealed on the cytological and molecular levels in malarial mosquito species from the Anopheles maculipennis complex. In this connection, investigation of the heterochromatin molecular composition in species of this complex is of interest.

[Analysis of sex chromosome homologies in representatives of the family Calliphoridae].

The distribution of sequences homologous to the Calliphora erythrocephala Mg. sex chromosome was studied in Protophormia terranovae R-D and Lucilia sp. The chromatin structure was found to be similar in regions containing homologous DNA sequences.

[Analysis of the spatial organization of the XL chromosome attachment site in nurse cell nuclei of the malaria mosquito Anopheles atroparvus].

The spatial position of the site of XL chromosome attachment to the nuclear envelope of ovarian nurse cells relative to the oocyte has been analyzed in the malaria mosquito Anopheles atroparvus. The XL chromosome attachment sites in the oocyte-nurse cell system of this species have been demonstrated to be orderly arranged, with the attachment sites in two out of three nurse cells in the same layer identically oriented relative to the oocyte.

[Intranuclear dynamics of chromosome 6 in nurse cells of Calliphora erythrocephala Mg. (Diptera: Calliphoridae)].

Intranuclear dynamics of chromosome 6 in nurse cell nuclei of Calliphora erythrocephala Mg. (Diptera: Calliphoridae) was studied. The 3D FISH method was used for the first time to study chromosome territories in highly polyploid nuclei whose chromosomes undergo morphological changes.

[Development of ovarioles and nurse cells cytoskeleton in Calliphora erythrocephala Mg. (Diptera; Calliphoridae)].

We have carried out the analysis of ovariole development and morphology in Calliphora erythrocephala Mg. (Diptera: Calliphoridae). It has been shown that several regions can be distinguished in C.

Molecular organization of heterochromatin in malaria mosquitoes of the Anopheles maculipennis subgroup.

Although heterochromatin makes up a significant portion of the malaria mosquito genome, its organization, function, and evolution are poorly understood. Sibling species of the Anopheles maculipennis subgroup, the European malaria mosquitoes, are characterized by striking differences in the morphology of pericentric heterochromatin; however, the molecular basis for the rapid evolutionary transformation of heterochromatin is not known. This study reports an initial survey of the molecular organization of the pericentric heterochromatin in nonmodel species from the A.

[Molecular cytogenetic analysis of DNA from pericentric heterochromatin of chromosome 2L of malaria mosquito Anopheles beklemishevi (Culicidae, Diptera)].

Using the method of microdissection of polytene chromosomes, followed by in situ hybridization, chromosomal localization of region-specific DNA probe from pericentic heterochromatin of chromosome 2L of Anopheles beklemishevi Stegnii et Kabanova was examined on polytene chromosomes of Anopheles atroparvus van Thiel, An. messeae Fall, and An. beklemishevi.

[Molecular-cytogenetic analysis of pericentromeric heterochromatin in ovarian nurse cells of Drosophila melanogaster subgroup species].

Evolutionary rearrangements of pericentromeric heterochromatin among Drosophila melanogaster subgroup species have been investigated. Region-specific DNA library from Drosophila orena ovarian nurse cell chromocenter has been obtained by microdissection of polytene chromosomes. The probe was localized on ovarian nurse cells chromosomes of D.

[The effects of inbreeding and low temperature on the pattern of chromosome synapsis in the ovarian nurse nuclei of Drosophila melanogaster strains].

The effects of inbreeding and low temperature on the pattern of homologous chromosome synapsis in ovarian nurse cell nuclei of Drosophila melanogaster strains were studied. Exposure to decreased temperature (16 degrees C) caused a noticeable increase in the rate of asynapses of homologous chromosomes, whereas this effect was insignificant for F30 inbreeding generation. Long-term inbreeding has a substantial effect on the relative positions of chromosomes in the nurse cell nuclei.

[Morpho-cytogenetic characteristic of the mosquito Anopheles artemievi (Diptera: Culicidae), a malaria vector from the complex maculipennis].

Detailed morphological analysis of all stages of the mosquito Anopheles artemievi Gordeev et al., 2005 is carried out. Characteristic traits that allow discriminating of An.

[Morphological and genetic characteristics of the malarial mosquito Anopheles beklemishevi (Diptera, Culicidae)].

The paper presents morphological and genetic data on the malarial mosquito Anopheles beklemishevi Stegnii et Kabanova, 1976. It also provides morphological accounts of eggs, age stage IV larvae, and, firstly, pupae and imagoes. The paper considers it possible to use cytogenetic and molecular markers to identify An.

[Evolutionary significance of chromosome architecture for epigenetic control of eukaryote development and phylogeny].

The significance of the spatial organization of chromosomes in germline tissue as a positional system controlling segregation in oogenesis is considered. The history of the problem is reviewed. The author's data on reorganization of chromosome structure in germline tissue considered in terms of systemic mutations are systematized.

[Localization of repetitive DNA sequences in the pericentromeric heterochromatin of malarial mosquitoes of the "Anopheles maculipennis" complex].

Distribution of eight fragments of conserved repetitive DNA from pericentromeric heterochromatin of chromosome 2 of Anopheles atroparvus has been investigated by in situ hybridization on polytene chromosomes of An. atroparvus and An. messeae.

[Visualization of chromosome territories in interphase nuclei of ovarian nurse cells in Calliphora erythrocephala Mg. (Diptera: Calliphoridae)].

Analysis of localization of chromosomes 2, 3, and 6 of Calliphora erythrocephala Mg. in ovarian nurse cell nuclei with different chromatin structure has shown that the regions of DNA probe hybridization reduced with increasing chromatin compaction. Hybridization of DNA probes of chromosomes 3 and 6 to secondary reticular nuclei demonstrated that chromosomes retain their territories in the nuclei when the chromatin acquires a reticular structure.

[Characterization and comparative analysis of DNA from the pericentric heterochromatin of chromosome 2 of Anopheles atroparvus V. Tiel (Culicidae, Diptera)].

The minilibrary containing DNA sequences from the diffuse pericentric heterochromatin from the right arm of Anopheles atroparvus V. Tiel (Culicidae, Diptera) chromosome 2 (2R) was generated by use of chromosome microdissection technique. Southern-blot hybridization of the minilibrary fragments with the labeled genomic DNA of A.

[Organization and differential staining of chromosomes from the endomitotic nucleus of trophocytes Calliphora erythrocephala (Diptera: Calliphoridae)].

The structure of primary polytene chromosomes and general architecture of nurse cell nuclei was studied in Calliphora erythrocephala using various methods of differential chromosome banding(G-, R-, C-banding; Ag- and DAPI staining), chromospecific DNA probes and fluorescence in situ hybridization. This analysis revealed differential compaction of particular chromosome regions. The localization of material of polytene chromosome 6 is retained after its rearrangement and the formation of the internal reticular structure of the nucleus.