Evaluation of possible pro- or antioxidative properties and of the interaction capacity with the microsomal cytochrome P450 system of different NMDA-receptor ligands and of taurine in vitro.

In the first part of the study possible additional antioxidative effects of various N-methyl-D-aspartate (NMDA)-receptor antagonists, some of which are used in the treatment of Parkinson's or Alzheimer's disease or as narcotic (dizocilpine, ketamine, budipine, memantine, amantadine, AP-5) were investigated in vitro in comparison to the respective agonists (NMDA, glutamate, aspartate, glycine) and the putative antioxidative amino acid taurine. For this purpose, effects on cytochrome P450 (P450) mediated oxidase functions in rat liver and brain microsomes were examined by measuring the influence on stimulated lipid peroxidation (LPO), H2O2 production, and lucigenin and luminol amplified chemiluminescence. Additionally, effects on rat whole blood chemiluminescence (WB-CL) were assessed.