Publications by authors named "Stefaniya Konstantinova Boneva"
Background: Retinal neovascularization (RNV) membranes can lead to a tractional retinal detachment, the primary reason for severe vision loss in end-stage disease proliferative diabetic retinopathy (PDR). The aim of this study was to characterize the molecular, cellular and immunological features of RNV in order to unravel potential novel drug treatments for PDR.
Methods: A total of 43 patients undergoing vitrectomy for PDR, macular pucker or macular hole (control patients) were included in this study.
Purpose: To decipher the transcriptional signature of macrophages of the human vitreous, also known as hyalocytes, and compare it to the profiles of other myeloid cell populations including human blood-derived monocytes, macrophages, and brain microglia.
Methods: This study involves a total of 13 patients of advanced age with disorders of the vitreoretinal interface undergoing vitrectomy at the University Eye Hospital Freiburg between 2018 and 2019. Vitreal hyalocytes were analyzed by fluorescence-activated cell sorting (FACS) and isolated as CD45CD11bCX3CR1Mat-Mac cells using a FACS-based sorting protocol.
Article Synopsis
- The study aimed to analyze the expression of HIF-1α and its effects on various types of tumors in the ocular adnexa to assess its role as a potential prognostic indicator.
- Researchers examined 49 patients with malignant tumors (like squamous cell carcinomas, non-Hodgkin lymphomas, and melanomas) and compared them with 30 patients with benign tumors, using methods like immunohistochemistry and transcriptomic analysis.
- Results showed that SCC tumors had significantly higher HIF-1α expression compared to benign tumors, and elevated HIF-1α levels correlated with a potentially worse prognosis for malignant cases, implicating its involvement in tumor growth and spread.