Publications by authors named "Stefania Torri"
Objectives: This study aimed to examine the efficacy of whole genome sequencing (WGS) in accurately predicting susceptibility profiles, potentially eliminating the need for conventional phenotypic drug susceptibility testing (pDST) for first-line antituberculosis drugs in routine tuberculosis diagnosis.
Methods: Over the period of 2017 to 2020, 1114 Mycobacterium tuberculosis complex isolates were collected with drug susceptibility testing conducted using the MGIT960 system and WGS performed for predicting drug resistance profiles. In addition, we implemented a new algorithm with an updated WGS workflow, omitting pan-susceptible strains from pDST.
Management of early-onset prosthetic joint infection: Case report and literature review.
J Clin Tuberc Other Mycobact Dis
May 2024
Article Synopsis
- - Nontuberculous mycobacteria are an uncommon but increasingly recognized cause of challenging infections in prosthetic joints, with only 17 documented cases in existing literature.
- - There are no established guidelines for treating these specific infections, making management difficult for healthcare providers.
- - The case of a 68-year-old woman with an early prosthetic joint infection is discussed, highlighting a successful tailored treatment approach and providing an overview of the available cases to help other physicians.
serogroup 1 (Lp1) sequence type (ST) 23 is one of the most commonly detected STs in Italy where it currently causes all investigated outbreaks. ST23 has caused both epidemic and sporadic cases between 1995 and 2018 and was analysed at genomic level and compared with ST23 isolated in other countries to determine possible similarities and differences. A core genome multi-locus sequence typing (cgMLST), based on a previously described set of 1,521 core genes, and single-nucleotide polymorphisms (SNPs) approaches were applied to an ST23 collection including genomes from Italy, France, Denmark and Scotland.
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- Detecting tuberculosis (TB) in cases of extrapulmonary infection is challenging due to low levels of bacteria and limited availability of fresh samples for traditional culture testing.
- A study evaluated homemade droplet digital PCR (ddPCR) assays using biopsies, comparing their effectiveness against culture methods on fresh and formalin-fixed, paraffin-embedded (FFPE) samples.
- Results showed that the IS6110 ddPCR assay had high sensitivity (98.5%) and accuracy (98.9%) for diagnosing TB compared to traditional methods, outperforming both the rpoB assay and other diagnostic techniques like Xpert and acid-fast bacilli smear microscopy.
Objectives: Rapid and reliable exclusion of invasive fungal infections (IFI) by markers able to avoid unnecessary empirical antifungal treatment is still a critical unmet clinical need. We investigated the diagnostic performance of a newly available β-d-Glucan (BDG) quantification assay, focusing on the optimisation of the BDG cut-off values for IFI exclusion.
Methods: BDG results by Wako β-glucan assay (lower limit of detection [LLOD] = 2.
Objectives: Mycobacterium xenopi is a nontuberculous mycobacterium (NTM) whose clinical diagnosis and drug susceptibility studies are frequently hampered by poor in vitro growth. Extending the culture incubation time from 42 days (common-standard) to 56 days could improve the likelihood of diagnosis and provide strains for phenotypic drug susceptibility profiling of this poorly studied but clinically relevant mycobacterium.
Methods: Time-to-positivity of mycobacterial cultures incubated for 56 days were analysed and compared.