Single-use porous thin film extraction with gas chromatography atmospheric pressure chemical ionization tandem mass spectrometry for high-throughput analysis of 16 PAHs.

The need for high throughput, reliable analytical methods for environmental analysis has driven our development of novel sampling technologies that can be used in microextraction devices and integrated into chromatographic systems for fast analysis. Here we report a new method of water analysis for 16 EPA priority polycyclic aromatic hydrocarbons (PAHs) that relies on a tailor made porous polymeric film for extraction with analysis by gas chromatography with atmospheric pressure chemical ionization-tandem mass spectrometry (APGC-MS/MS). The extraction device is user friendly, single-use and gives exhaustive extraction from a relatively small volume (20 mL).

Tandem mass spectrometry determination of the putative structure of a heterogeneous mixture of Lipid As isolated from the lipopolysaccharide of the Gram-negative bacteria Aeromonas liquefaciens SJ-19a.

Rationale: We report herein the electrospray ionization mass spectrometry (ESI-MS) and low-energy collision-induced dissociation tandem mass spectrometry analysis (CID-MS/MS) of a mixture of lipid As isolated from the rough lipopolysaccharide (LPS) of the mutant wild strain of the Gram-negative bacteria Aeromonas liquefaciens (SJ-19a, resistant) grown in the presence of phages. The interaction between the phages and the Gram-negative bacteria regulates host specificity and the heterogeneity of the lipid A component of the LPS.

Methods: The heterogeneous mixture of lipid As was isolated by the aqueous phenol method from the LPS of the rough wild strain of Gram-negative bacteria Aeromonas liquefaciens (SJ-19a).