Efficient immobilization of mushroom tyrosinase utilizing whole cells from Agaricus bisporus and its application for degradation of bisphenol A.

A simple and efficient procedure for preparation and immobilization of tyrosinase enzyme was developed utilizing whole cells from the edible mushroom Agaricus bisporus, without the need for enzyme purification. Tyrosinase activity in the cell preparation remained constant during storage at 21 °C for at least six months. The cells were entrapped in chitosan and alginate matrix capsules and characterized with respect to their resulting tyrosinase activity.

Conclusions on plutonium separation from atmospheric krypton-85 measured at various distances from the Karlsruhe reprocessing plant.

For wide-area atmospheric monitoring, krypton-85 is the best indicator for clandestine plutonium separations. The detection and false alarm rates were determined from weekly samples at five different distances from the Karlsruhe reprocessing plant between 1985 and 1988. The detection rate for the separation of 4 kg of plutonium per week was found to be as high as 80-90% at a distance of less than 1 km, 70% at 5 km, 40% at 39 km, and 15% at 130 km.