Publications by authors named "Stefan Semerdzhiev"
Article Synopsis
- Microorganisms produce extracellular polymeric substances (EPS) which have diverse industrial applications, especially those from cyanobacteria due to their complexity.
- The study examined how different culture media affect the quantity and composition of EPS in the cyanobacterium Synechocystis sp. PCC 6803, finding that early growth phases yield more EPS per cell, but overall amounts accumulate later.
- Results indicated that lower magnesium boosts production per cell but reduces total sugar content, and specific sugars correlate with media components; a potential biosynthetic pathway and protective function for xylose-rich EPS were proposed based on RNA sequencing.
The genetic engineering of microbial cell factories is a sustainable alternative to the chemical synthesis of organic compounds. Successful metabolic engineering often depends on manipulating several enzymes, requiring multiple transformation steps and selection markers, as well as protein assembly and efficient substrate channeling. Naturally occurring fusion genes encoding two or more enzymatic functions may offer an opportunity to simplify the engineering process and to generate ready-made protein modules, but their functionality in heterologous systems remains to be tested.
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