Rapid prototyping of electrochromatography chips for improved two-photon excited fluorescence detection.

In the present study, we introduce two-photon excitation at 532 nm for label-free fluorescence detection in chip electrochromatography. Two-photon excitation at 532 nm offers a promising alternative to one-photon excitation at 266 nm, as it enables the use of economic chip materials instead of fused silica. In order to demonstrate these benefits, one-photon and two-photon induced fluorescence detection are compared in different chip layouts and materials with respect to the achievable sensitivity in the detection of polycyclic aromatic hydrocarbons (PAHs).

Integrating continuous microflow reactions with subsequent micropreparative separations on a single microfluidic chip.

A current challenge in continuous flow chemistry using microfluidic devices is the combination with a micropreparative separation technique for online product clean-up and analysis. Herein we describe an approach for seamless on-chip integration of continuous flow reactors with a downstream separation functionality based on free-flow electrophoresis. A prototype device was fabricated via liquid phase lithography in a one-step procedure.

Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing.

We immobilized adherent human embryonic kidney (HEK) cells--which are able to trace adenosine triphosphate (ATP)--inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.

Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing.

Functional microfluidic free-flow electrophoresis chips with integrated fluorescent pH sensors are presented. Polyethylene glycol based structures were fabricated that allowed for integration of both functions on a single microchip. Microchips were applied in free-flow isoelectric focusing of model compounds and proteins with on-line monitoring of pH during microscale electrophoretic separation.

Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips.

We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated.