The use of affinity matrices in the purification of inhibin from bovine follicular fluid.

The protein associated with inhibin-like activity in bovine follicular fluid was purified 80- to 120-fold after successive adsorptions on different affinity matrices, i.e. Matrex gel red A, phenyl sepharose, omega-aminohexyl agarose and concanavalin-A sepharose.

Retinoscopy and chromatic aberration.

The origin of the apparent farsightedness as revealed by retinoscopy in smaller eyes was investigated by using monochromatic retinoscopy on wild rabbits. Our results indicate that a combination of long wavelength light and chromatic aberration of the subject's dioptrics is the major source of this artifact.

Clinical course in 28 unselected patients with aplastic anaemia treated with anabolic steroids.

Twenty-eight unselected patients with histologically proven aplastic anaemia were electively treated with anabolic steroids (75-150 mg orally q.d.) Additional supportive treatment with blood cell components and antibiotics was given if indicated.

Serological grouping of virulent and avirulent strains of the lobster pathogen Aerococcus viridans.

Virulent strains of Aerococcus viridans (formerly Gaffkya homari) are the aetiologic agents of gaffkemia, a septicaemic disease of the American lobster (Homarus americanus). The virulent and avirulent forms of this bacterium, previously thought to be taxonomically indistinguishable, have been differentiated by serological studies. Antisera were produced in rabbits using autoclaved bacteria as antigens.

Clinical course and survival after elective splenectomy in 19 patients with primary myelofibrosis.

Nineteen patients with primary myelofibrosis and myeloid metaplasia (MMM), who fulfilled well-defined criteria, underwent elective splenectomy as soon as the diagnosis was confirmed. Nine patients developed postoperative complications such as intra-abdominal bleeding (three), rupture of the inferior vena cava (one), ascites (two), Australia antigen-positive hepatitis (three), mesenteric artery thrombosis (one) and pneumonia (one). One patient died within a month of the operation due to secondary intra-abdominal infection.

RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium.