Strategies to enhance the efficacy of FGF2-based therapies for skin wound healing.

Basic fibroblast growth factor (FGF2 or bFGF) is critical for optimal wound healing. Experimental studies show that local application of FGF2 is a promising therapeutic approach to stimulate tissue regeneration, including for the treatment of chronic wounds that have a low healing potential or are characterised by a pathologically altered healing process. However, the problem of low efficiency of growth factors application due to their rapid loss of biological activity in the aggressive proteolytic environment of the wound remains.

Distinct calcium regulation of TRPM7 mechanosensitive channels at plasma membrane microdomains visualized by FRET-based single cell imaging.

Transient receptor potential subfamily M member 7 (TRPM7), a mechanosensitive Ca channel, plays a crucial role in intracellular Ca homeostasis. However, it is currently unclear how cell mechanical cues control TRPM7 activity and its associated Ca influx at plasma membrane microdomains. Using two different types of Ca biosensors (Lyn-D3cpv and Kras-D3cpv) based on fluorescence resonance energy transfer, we investigate how Ca influx generated by the TRPM7-specific agonist naltriben is mediated at the detergent-resistant membrane (DRM) and non-DRM regions.

Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells.

Extracellular vesicles derived from mesenchymal stem cells (MSCs) represent a novel approach for regenerative and immunosuppressive therapy. Recently, cytochalasin B-induced microvesicles (CIMVs) were shown to be effective drug delivery mediators. However, little is known about their immunological properties.

Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay.

The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in the same cell sample using a dual resazurin/sulforhodamine B assay, eliminating the variation associated with cell seeding and excessive manipulations in assays that test different cell samples across plates.

[Nitrogen oxide is involved in the regulation of the Fe-S cluster assembly in proteins and the formation of biofilms by Escherichia coli cells].

The functions of nitrogen oxide (NO) in the regulation of the reversible processes of Fe-S cluster assembly in proteins and the formation of Escherichia coli biofilms have been investigated. S-nitrosoglutathione (GSNO) and crystalline nitrosyl complexes of iron with sulfur-containing aliphatic ligands cisaconite (CisA) and penaconite have been used as NO donors for the first time. Wild-type E.

[Frequency of detection the of Brugada syndrome signs in the course of ECG registration].

The aim of the study was to assess prevalence of the Brugada syndrome during carrying out ECG in the conditions of medico-sanitary division of a large industrial enterprise. Signs of the Brugada syndrome were found on 20 of 42779 ECGs (0.047%) registered during 1 year in patients aged 22-68 years.

[The immune response of viral hepatitis patients who use narcotic preparations].

The characteristics of cell-mediated and humoral immunity were studied in 611 patients with different etiological forms of acute virus hepatitides (HB, HC, HB + C, HC + HBsAg). As the result of the systematic abuse of psychoactive preparations, introduced by intravenous injection, in 166 patients (27.2%) drug addiction developed, ehile 445 (72.

[The prevalence of hepatitis B and C markers among narcotic users].

611 patients with acute parenteral virus hepatitis (VH) were studied with a view to find out markers indicating the presence of hepatitis B and hepatitis C virus infection (HB, HC, HB + C, HC + HBsAg). Of these, 166 patients (27.2%) systematically used narcotic drugs intravenously.

[The dynamics of the basic factors of nonspecific resistance in North Asian tick-borne typhus].

At the acute period of tick-borne typhus an increase in the activities of the main nonspecific resistance factors of the body, and particularly lysozyme, betalysins, complement and properdin, is observed. During convalescence the concentration of lysozyme normalizes and the content of betalysins remains sufficiently high, this indicating that, even in the absence of clinical sings of the disease, the disturbances in homeostasis persist. A decrease in the levels of complement and properdin during convalescence is seemingly linked with their consumption for the formation of immune complexes, eliminated from the body.

[The clinico-epidemiological characteristics of the course and diagnosis of tick-borne rickettsiosis in northern Asia].

The study of tick-borne rickettsiosis in Northern Asia has made it possible to determine the clinico-epidemiological features of the modern course of this disease, which should be taken into consideration for the timely diagnosis of this infection. The laboratory verification of the diagnosis should be carried out, as recommended by the authors, in the agar precipitation and leukocytosis tests in their modification.