Invasiveness of Listeria monocytogenes strains isolated from animals in Poland.

Animals are important reservoir of Listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of L. monocytogenes strains of animal origin is very scarce.

A 34-year retrospective study of equine viral abortion in Poland.

The purpose of the present review was a comparison of the abortions caused by EAV and EHV-1 viruses over the 34 years. A total of 452 tissues samples from aborted fetuses (347) or foals (105) stillborn or newborn that died within 72 hours were investigated. The material for the examinations came from different farms located throughout Poland.

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells.

A total of 100 Malassezia pachydermatis strains recovered from skin and mucosal membranes of dogs were evaluated for their adhesive properties. Two types of growth, related to colony morphology on Sabouraud agar, were observed (type I and II). The mean number of fungal cells attaching to canine buccal epithelial cells was found to be 17.

Comparison of the biopsy and cytobrush techniques for diagnosis of subclinical endometritis in mares.

Background: Endometritis is a major cause of infertility in the mare. Therefore, the diagnosis of this disease is very important in veterinary practice. The objective of this study was to compare bacteriological and cytological results obtained from the mare uterus using biopsy (EB) and cytobrush (CB) techniques and relating these findings to the presence of polymorphonuclear cells (PMNs) in endometrial tissue as the gold standard for detection of endometritis.

Polymerase chain reaction-based identification of clinically relevant Pasteurellaceae isolated from cats and dogs in Poland.

A set of polymerase chain reaction (PCR) assays for identification of the most important Pasteurellaceae species encountered in cats and dogs were developed. Primers for Pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. Primers specific to Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (sodA) and those specific to [Haemophilus] haemoglobinophilus on species-specific sequences of the 16S ribosomal RNA gene.

Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences.

A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group.

Typing of Yersinia Enterocolitica Isolates by ITS profiling, REP- and ERIC-PCR.

Thirty-five Yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (ITS) profiling, REP- and ERIC-PCR. ERIC-PCR revealed the presence of seven different genotypes. Amplification of the 16S-23S rDNA spacer region by ITS profiling gave similar results with nine different genotypes.

[Level of macrophages and lymphocytes reacting with nitro-tetrazole blue (NBT) in mice infected with Yersinia enterocolitica].

The study was aimed at investigation of reducing ability of nitrotetrazol blue (NBT) by peritoneal fluid macrophages and spleen and mesenteric lymph node during experimental yersiniosis. Investigations were carried on BALB/c mice infected perorally by the dose of 10(6) of live, virulent cells of Y. enterocolitica.

[Serological studies of human Yersinia enterocolitica infections].

Sera of 1283 patients from hospitals and medical centers of Wrocław were examined for Y. enterocolitica antibodies. In 29% examined sera anti-Y.

Influence of immunisation with enteropathogenic and non-enteropathogenic Escherichia coli on the occurrence of loop dilation in rabbits.

The protective effects of high levels of O115 antibodies and K88 antibodies in the sera of hyperimmunized rabbits on the intestinal loop dilation elicited by live cultures of Escherichia coli O115: K88:H39 and its enterotoxins were examined. K88 antibodies did not prevent fluid accumulation when live E coli O115:K88:H39 or its enterotoxins were injected into ligated loops in rabbits actively immunised with K12:K88 or when K88 antiserum was mixed directly with the challenge shortly before injection into the loops. In two of six rabbits immunised with heat-killed O115 E coli the live strain failed to elicit a fluid response and in two others the response was reduced.