A statistical evaluation of the binding of ferritin-conjugated lectins to the surface of rat cells. Topographical variations as a function of lectin concentration and cell type.

Statistical procedures were utilized to evaluate the concentration dependence of labeling by ferritin-conjugated lectins on four different rat cells: hepatocytes, normal thymocytes, Friend virus-induced rat tumor cells and feline sarcoma virus-induced rat sarcoma cells. Labeling by ferritin conjugates of concanavalin A, wheat germ agglutinin and Ricinus communis agglutinins I and II was quantitated by counting the number of ferritin granules on 600 Angstrom membrane segments. Relationships between the arithmetic means and variances for sample populations from each cell and ferritin-lectin combination were used to define four types of topographical distributions: uniform/ordered, uniform/random, random and clustered.

Hemosuccus pancreaticus secondary to ruptured splenic artery aneurysm.

A case is presented of a ruptured splenic artery aneurysm communicating with the main pancreatic duct which resulted in obscure upper gastrointestinal bleeding. Bipolar ligation of the splenic artery and excision of the pseudoaneurysm was curative. The location of the splenic artery makes it especially vulnerable to aneurysmal formation in patients with pancreatitis.

Initial treatment of sigmoid volvulous by colonoscopy.

The initial management of acute, nonstrangulated sigmoid volvulous is to attempt proctosigmoidoscopic, rectal tube, or barium enema reduction and evacuation. If unsuccessful emergency surgery is necessary. The flexible colonoscope offers an additional therapeutic modality to effectuate preoperative reduction of the twisted sigmoid colon if attempts with conventional methods fail.

Treatment of hiatal hernia with esophagitis in a patient using esophageal speech after laryngectomy.

Severe reflux esophagitis occurring after total laryngectomy in a patient who has learned to communicate by esophageal speech represents a unique problem in management. It was anticipated that an antireflux operation might interfere with esophageal speech. A Nissen fundoplication resulted in complete resolution of signs and symptoms of reflux esophagitis without compromising esophageal speech.

Carbohydrate transport in Moniliformis dubius (Acanthocephala). III. Post-absorptive fate of fructose, mannose, and galactose.

The initial metabolism of fructose, mannose, and galactose in Moniliformis dubius (M. moniliformis; Acanthocephala) was examined following brief aerobic incubations in 14C-labeled substrate. The pattern of incorporation of radioactivity from 14C-fructose and 14C-mannose into intermediates of hexose metabolism was indistinguishable from that reported elsewhere for the initial post-absorptive metabolism of 14C-glucose under comparable conditions; these hexoses were phosphorylated rapidly following their absorption, and much of the radioactivity absorbed as mannose or fructose was recovered in the nonreducing disaccharide trehalose [alpha-D-glucopyranosyl-(1 leads to 1)-alpha-D-glucopyranoside].

Production of monoclonal antibodies against a cell surface concanavalin A binding glycoprotein.

Concanavalin A-binding (Con-A)-binding cell surface glycoproteins were isolated, via Con A-affinity chromatography, from Triton X-100-solubilized Chinese hamster ovary (CHO) cell plasma membranes. The Con A binding glycoproteins isolated in this manner displayed a significantly different profile on sodium dodecyl sulfate--polyacrylamide gels than did the Triton-soluble surface components, which were not retarded by the Con-A-Sepharose column. [125I]-Con A overlays of the pooled column fractions displayed on sodium dodecyl sulfate--polyacrylamide gel electro-phoresis (SDS-PAGE) demonstrated that there were virtually no Con A receptors associated with the unretarded peak released by the Con A-Sepharose column, whereas the material which was bound and specifically eluted from the Con A-Sepharose column with the sugar hapten alpha-methyl-D-mannopyranoside contained at least 15 prominent bands which bound [125I]-Con A.

Diagnosis of occult familial medullary carcinoma of the thyroid using pentagastrin.

Medullary carcinoma of the thyroid is unique in that it can often be diagnosed solely on the basis of abnormally high basal serum thyrocalcitonin levels. Two brothers described in this report were found to have normal basal serum thyrocalcitonin levels when examined after another family member died from metastatic medullary carcinoma of the thyroid. Pentagastrin, a synthetic analog of gastrin, was subsequently administered to these two high-risk relatives and found to stimulate the release of thyrocalcitonin to abnormally high levels.

Prolongation of survival of rat parathyroid allografts by enhancing serum and tissue culture.

To determine whether survival of allografted rat parathyroid tissue could be enhanced either by the administration of antidonor serum (enhancing serum) or by tissue culture of the graft, parathyroids from Lewis x Brown Norway F1 (LBNF1) rats were transplanted beneath the fascia and between hamstring muscle fibers of hypocalcemic parathyroidectomized Lewis recipients (serum calcium 4.3 +/- 0.13mg%--mean +/- SEM).

Acute jejunogastric intussusception.

Our patient represents an example of acute retrograde jejunograstric intussusception with the additional finding of a bleeding jejunal ulcer in the intussusception. The ulcer may have been etiological in precipitating the intussusception, or a consequence of mucosal sloughing in the hemorrhagic intussusception. Early diagnosis and immediate surgical therapy resulted in an uneventful recovery.

Carbohydrate transport in Moniliformis dubius (Acanthocephala). I. The kinetics and specificity of hexose absorption.

The uptakes of 14C-glucose, -2-deoxyglucose, -mannose, -N-acetylglucosamine, -3-0-methylglucose, -fructose, and -galactose by female Moniliformis dubius were nonlinear, saturable functions of hexose concentration. Kinetic and inhibition studies indicated that glucose and 2-deoxyglucose were absorbed via a single common transport locus. Mannose, N-acetylglucosamine, 3-0-methylglucose, fructose, and galactose (in decreasing order of effectiveness) inhibited the uptake of glucose in a completely competitive manner; their absorptions appeared to be mediated by the glucose transport locus and, to some degree, by one or more additional transport systems.

Erythrocyte filterability and lysosomal enzymes in patients requiring cardiopulmonary bypass.

Erythrocyte filterability, an index of deformability, and lysosomal enzyme activity were studied in 34 patients requiring cardiopulmonary bypass. In addition fresh human erythrocytes were analyzed after incubation for filterability changes after exogenous lysosomal enzymes were added to the suspension medium. The results showed statistically significant elevations of the filterability index (decrease in filterability of the red cells) after 10 minutes on cardiopulmonary bypass as well as postoperatively.