Evaluation of cleansing methods for previously worn prostheses.

Statement Of Problem: Although there are many product claims that address the issue of denture sanitization, controlled scientific studies on previously worn dentures have not been performed.

Purpose: The purpose of this study was to evaluate procedures directed at sanitizing previously worn contaminated dentures from two regions of the United States.

Materials And Methods: This study examined 51 previously worn dentures from two regions.

Evaluation of microbial flora found in previously worn prostheses from the Northeast and Southwest regions of the United States.

Statement Of Problem: Denture-induced stomatitis is a recognized clinical challenge. The responsible microorganisms have not been delineated and may differ among regions of the United States.

Purpose: The purpose of this study was to identify the microorganisms found in dentures from 2 geographic regions.

A stability-indicating HPLC method for the determination of glucosamine in pharmaceutical formulations.

A stability-indicating high performance liquid chromatographic (HPLC) method was developed for the assay of glucosamine in bulk forms and solid dosage formulations. The HPLC separation was achieved on a Phenomenex Luna amino column (150 mm x 4.6 mm, i.

Structural identification of nonvolatile dimerization products of glucosamine by gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and nuclear magnetic resonance analysis.

The degradation profile of glucosamine bulk form stressed at 100 degrees C for 2 h in an aqueous solution was studied. Column chromatography of acetylated product mixture led to isolation of two pure compounds (1b and 2b) and a mixture of at least three isomers (3b). 1a and 2a were identified as 5-(hydroxymethyl)-2-furaldehyde (5-HMF) and 2-(tetrahydroxybutyl)-5-(3',4'-dihydroxy-1'-trans-butenyl)pyrazine, respectively, by utilizing a variety of analytical techniques, such as GC-MS, LC-MS, on-line UV spectrum, (1)H and (13)C NMR, and DEPT, as well as (1)H-(1)H COSY.