Enhancement of analyte detectability in isotachophoresis exploiting spectrophotometric diode-array detection with chemometric data processing.

The potentialities of capillary ITP combined with diode-array detection (DAD) with subsequent chemometric data processing have been investigated in this work. A series of different migration configurations were created using model analytes, interferents and appropriate spacers. Special attention has been paid not only to constituents migrating in fully developed ITP zones but also to the spike mode of ITP migration.

Determination of orotic acid in urine by capillary zone electrophoresis in tandem-coupled columns with diode array detection.

Analytical potentialities of capillary zone electrophoresis in the separation system with tandem-coupled columns to the spectral identification and determination of orotic acid (OA) in urine by diode array detection (DAD), coupled to the separation system via optical fibers, were investigated. A very significant "in-column" clean-up of OA from urine matrix was reached in the separation stage of the tandem by combining a low pH (2.8) with complexing effects of electroneutral agents [alpha- and beta-cyclodextrins, poly(vinylpyrrolidone) and 3-(N,N-dimethyldodecylammonio)propanesulfonate].

Capillary zone electrophoresis in wide bore capillary tubes with fiber-coupled diode array detection.

This feasibility study deals with the use of a wide bore (320 microm I.D.) capillary tube for the detection and identification of capillary zone electrophoresis (CZE) analytes by optical fiber-coupled diode array detection.