A Novel Method for Fragmentation Studies in Particle Therapy: Principles of Ion Identification.

Purpose: In carbon ion beam radiation therapy, fragmentation processes within the patient lead to changes in the composition of the particle field with increasing depth. Consequences are alterations of the resulting dose distribution and its biological effectiveness. To enable accurate treatment planning, the characteristics of the ion spectra resulting from fragmentation processes need to be known for various ion energies and target materials.

Jacobsen catalyst as a cytochrome P450 biomimetic model for the metabolism of monensin A.

Monensin A is a commercially important natural product isolated from Streptomyces cinnamonensins that is primarily employed to treat coccidiosis. Monensin A selectively complexes and transports sodium cations across lipid membranes and displays a variety of biological properties. In this study, we evaluated the Jacobsen catalyst as a cytochrome P450 biomimetic model to investigate the oxidation of monensin A.

Biosynthesis of colabomycin E, a new manumycin-family metabolite, involves an unusual chain-length factor.

Colabomycin E is a new member of the manumycin-type metabolites produced by the strain Streptomyces aureus SOK1/5-04 and identified by genetic screening from a library of streptomycete strains. The structures of colabomycin E and accompanying congeners were resolved. The entire biosynthetic gene cluster was cloned and expressed in Streptomyces lividans.

In vitro metabolism of monensin A: microbial and human liver microsomes models.

1. Monensin A, an important antibiotic ionophore that is primarily employed to treat coccidiosis, selectively complexes and transports sodium cations across lipid membranes and displays a variety of biological properties. 2.

New carbasugars from Streptomyces lincolnensis.

Two new carbasugars (9 and 10) were isolated from Streptomyces lincolnensis DSM 40355 along with streptol (valienol, 8), gabosine I (valienone, 14), and glucosylglycerate. The reported (1)H and (13)C assignments are based on 1D ((1)H, (13)C, 1D-TOCSY, homodecoupling) and 2D (gCOSY, J-resolved, TOCSY, ROESY, gHSQC, gHMBC) NMR techniques and electrospray ionization FT mass spectrometry (ESI FTMS).

HPLC-fluorescence detection method for determination of key intermediates of the lincomycin biosynthesis in fermentation broth.

The biosynthetic pathway of the clinically important antibiotic lincomycin is not known in details. The precise knowledge of the lincomycin biosynthesis is a prerequisite for generation of improved derivatives by means of combinatorial genetics. Methods allowing determination of the key intermediates are very important tools of the pathway investigation.

Small-scale in vivo imaging of soft tissues by radioscopy using single X-ray photon counting.

A method has been developed for routine laboratory visualisation of small-scale soft tissue by means of transmission X-ray radioscopy and tomography. Using termites as models, imaging quality with a spatial resolution of about 3 mum was achieved and 3D tomographic reconstruction was demonstrated. A termite worker individual was visualized before and after its metamorphosis towards the soldier caste.

Extracellular carbohydrate metabolites from Streptomyces coelicolor A3(2).

A new ribose trisaccharide, alpha-Ribf-(1-->2)-alpha-Ribf-(1-->3)-alpha-Ribf (1), was isolated together with 5-O-(alpha-mannosyl)-myo-inositol (2), 2-O-(alpha-mannosyl)-myo-inositol (3), trehalose (4), and d-ribulose (5) from a submerged cultivation of Streptomyces coelicolor A3(2). The structures of these compounds were elucidated by spectroscopic and chemical methods. Concentrations of these compounds in the medium were in the range from 0.