Suffering for science: the effects of implicit social demands on response to experimentally induced pain.

In two experiments subjects rated their pain during baseline trials of cold pressor and finger pressure pain. After various instructional treatments, they were posttested with these same stimuli. As in previous studies, we found in both experiments that coping suggestions significantly reduced reported pain.

Localization and function of myocardial lipolysis.

Mobilization of triacylglycerol stored in heart cells is accomplished by the combined action of lysosomal (acid) lipase and microsomal monoacylglycerol lipase or carboxylesterase. Non(heparin)-releasable neutral or alkaline lipase is similar to non(readily)-releasable lipoprotein lipase (LPL). The enzyme is mainly localized extracellularly.

Computerized tomography in carcinoma of the prostate: treatment volume and morbidity in radiotherapy.

Fifty-five patients with adenocarcinoma of the prostate were treated with external beam irradiation. In 31 of them conventional localization was used and in 24 computerized tomography was used in the localization of the treatment volume. In these 24 patients we see a larger treatment volume with a wider distribution.

The treatment of temporomandibular joint syndrome through control of anxiety.

Following a negative experience with general anesthesia, a 20-yr-old woman developed anxiety and an inability to relax concomitant with temporomandibular joint dysfunction and pain syndrome. Systematic countering of anxiety by relaxation successfully removed her anxiety and led to a complete resolution of her symptoms. Follow-up at 16 months indicated maintenance of treatment gains and no recurrence of the symptoms during the previous 12 months.

Hydrogen oxidation and nitrogen fixation in rhizobia, with special attention focused on strain ORS 571.

In this survey we describe the influence of hydrogen oxidation on the physiology of Rhizobium ORS 571. The presence of hydrogen is required for the synthesis of hydrogenase. Carbon substrates do not repress the synthesis of hydrogenase.

Comparison of heparin-releasable lipase and tissue neutral lipase activity of rat heart.

The alkaline, heparin-releasable lipoprotein lipase (LPL) activity of isolated, perfused rat hearts was compared with the residual neutral lipase (NL) activity detectable in the post nuclear supernatant (PNS) from a tissue homogenate. Both enzyme activities were increased by serum, heparin and apolipoprotein CII, inhibited by high salt concentrations and by immunotitration with an anti-LPL gamma-globulin fraction. Protamine sulphate from saline liver inhibited LPL activity and the NL activity only in the absence of serum.

The components of the carbon monoxide diffusing capacity in man dependent on alveolar volume.

The effect of alveolar volume (VA) on diffusing capacity for carbon monoxide (DL), membrane conductance (Dm) and pulmonary capillary blood volume (Qc) was investigated in 39 normal volunteers to study alveolar membrane expansion and capillary volume recruitment. DL/VA was related to alveolar volume breathing air and 95% oxygen respectively. Both relations appeared to be linear with a negative slope and were used to calculate Dm and Qc as a function of VA.

The effect of excess (acyl) carnitine on lipid metabolism in rat heart.

During Langendorff perfusion of rat hearts with Intralipid, the resulting fat accumulation in the hearts can be inhibited by the addition of 5 mM L-carnitine to the perfusion medium. The mechanism of this phenomenon is probably the inhibition of lipid accumulation in the heart by acylcarnitine rather than stimulation of fatty acid oxidation by excess carnitine addition. Palmitoylcarnitine was found to stimulate trioleoylglycerol hydrolysis at neutral pH in heart homogenates, when it was tested in the presence of relatively much protein.

Dual localization of lipoprotein lipase in rat heart. Its relationship to chylomicron degradation.

Rat hearts were perfused retrogradely using a modified technique that allows the separate collection of coronary (Qrv) and interstitial (Qi) effluents. Evidence is presented that Qrv contains products from the coronary vasculature and that Qi contains products arising from cardiac myocytes. Heparin perfusion of rat hearts led to a release of lipolytic activity in Qrv and Qi which was characterized as lipoprotein lipase (LPL).

Intracellular origin and regulation of endogenous lipolysis in rat heart.

The rate of glycerol release from isolated, perfused rat hearts was used as an index for endogenous lipolysis. Pharmacological and metabolic interventions were performed in order to obtain information about the intracellular site of action and regulation of tissue triglyceride (TG) hydrolysis in heart. It proved that endogenous lipolysis probably is of lysosomal origin.

Intracoronary infusion of small doses of nifedipine lowers regional myocardial O2-consumption without altering regional myocardial function.

Intracoronary infusion of low doses (0.1-0.3 microgram X kg-1) of nifedipine caused dose-dependent decreases in regional myocardial O2-consumption, without significant changes in any of its major global hemodynamic determinants: heart rate, left ventricular systolic and end-diastolic pressure and maxLVdP/dt.

Comparative study of chylomicron and fatty acid utilization in small intestine and heart.

Chylomicrons were isolated from the urine of rats after a surgical procedure in which the cysterna chyli was connected with the right ureter. The fatty acids of the chylomicrons served as a respiratory substrate for rat heart and not for rat small intestine during in vitro vascular perfusions. The reason for the absence of chylomicron utilization in small intestine was found to be the virtual absence of lipoprotein lipase from this organ.

Control of lipolysis in triglyceride-enriched rat hearts.

Hormonal and metabolic regulation of endogenous triglyceride hydrolysis was studied in triglyceride-enriched hearts obtained from rats fed 3 days with a trierucate-rich diet. Endogenous lipolysis was determined by measuring glycerol release during in vitro perfusion of the hearts. It appeared that there was a direct relation between the contractile state of the heart, the rate of glycerol release in the coronary effluent and the Ca2+ concentration in the perfusion medium.