Intravenous anti- IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia.

Ventilator associated pneumonia (VAP) caused by is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the investigation of novel therapies. Specific polyclonal anti- IgY-antibodies (IgY) might be effective for VAP caused by The objective of this study was to investigate if intravenous IgY decreases the lower airway concentration of in VAP.

Bronchially instilled IgY-antibodies did not decrease pulmonary p. aeruginosa concentration in experimental porcine pneumonia.

Background: P. aeruginosa possesses antibiotic resistance, making treatment difficult. Polyclonal anti-P.

The time course of calprotectin liberation from human neutrophil granulocytes after and endotoxin challenge.

Plasma calprotectin has previously been reported as a biomarker for sepsis. The aim of the present study was to elucidate the kinetics of calprotectin release from neutrophils exposed to and endotoxin. Whole blood samples were exposed to bacteria or endotoxin .

Correction to: Inhalation of specific anti-Pseudomonas aeruginosa IgY antibodies transiently decreases P. aeruginosa colonization of the airway in mechanically ventilated piglets.

Following publication of the original article [1], the authors flagged that an incorrect piece of data is given in the Materials and Methods section of the article.

Inhalation of specific anti-Pseudomonas aeruginosa IgY antibodies transiently decreases P. aeruginosa colonization of the airway in mechanically ventilated piglets.

Background: P. aeruginosa is a pathogen frequently resistant to antibiotics and a common cause of ventilator-associated pneumonia (VAP). Non-antibiotic strategies to prevent or treat VAP are therefore of major interest.

IgY stability in eggs stored at room temperature or at +4°C.

1. The aim of this study was to investigate the stability of immunoglobulin-Y (IgY) in stored eggs from immunised hens. 2.

Alkylaminopropenones and alkylamino-propenoates as efficient and versatile synthons in microwave-assisted combinatorial synthesis.

A simple and fast one-pot method using microwave irradiation for the synthesis of a number of small libraries of diverse heterocycles is described in this paper. The two-step one-pot method includes the formation of alkylaminopropenones or alkylaminopropenoates in 5 min at 180 degrees C and a subsequent treatment with dinucleophiles for 3 to 5 min at 150 degrees C to 180 degrees C to form a variety of biologically interesting heterocycles in a cascade-type reaction. The combination of combinatorial chemistry and microwave-assisted synthesis was found to be very efficient.

Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods.

Egg yolk is an important source of antibodies. The biggest obstacle for isolation of chicken antibodies (IgY) is the removal of lipids, which are present in abundance in egg yolk. We have used a two-phase system to separate egg yolk.

Chicken antibodies: a clinical chemistry perspective.

The chicken immune system has been studied for many years and these studies have contributed substantially to our understanding of the fundamental concepts of immunology and the development of different immunoglobulin classes. It is thus surprising that only a small fraction of the antibodies presently used in laboratories are of avian origin. A laying hen produces more yolk antibodies than a rabbit can produce during the same time period, and the animal care costs are lower for the chicken compared to the rabbit.