Publications by authors named "Stacey M Bailey"
is a highly virulent, multidrug-resistant pathogen that causes significant morbidity and mortality in hospitalized patients and is particularly devastating in patients with cystic fibrosis. Increasing antibiotic resistance coupled with decreasing numbers of antibiotics in the developmental pipeline demands novel antibacterial approaches. Here, we tested peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), which inhibit translation of complementary mRNA from specific, essential genes in PPMOs targeted to , , and , inhibited growth in many clinical strains and activity of PPMOs could be enhanced 2- to 8-fold by the addition of polymyxin B nonapeptide at subinhibitory concentrations.
View Article and Find Full Text PDFObjectives: The objective of this study was to test the efficacy of an inhibitor of the New Delhi metallo-β- lactamase (NDM-1). Inhibiting expression of this type of antibiotic-resistance gene has the potential to restore antibiotic susceptibility in all bacteria carrying the gene.
Methods: We have constructed a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) that selectively inhibits the expression of NDM-1 and examined its ability to restore susceptibility to meropenem in vitro and in vivo .
Article Synopsis
- * A new method has been developed that uses antisense oligomers to inhibit a key resistance gene in E. coli, allowing for lower doses of antibiotics to effectively inhibit bacterial growth.
- * This strategy not only enhances the effectiveness of existing antibiotics but also demonstrates compatibility with antibiotic combinations that usually struggle to work together, without harming human cells.