Publications by authors named "Staberg B"

Vitamin A and its derivatives (retinoids) exert modulatory effects on epithelial differentiation and are used therapeutically against skin cancers, but the role of dietary vitamin A in ultraviolet (UV)-induced carcinogenesis is far from clear. To study this process, 220 hairless mice were given diets containing low (0.3-0.

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Background: Data from open studies suggest that ranitidine has a beneficial effect on psoriasis and is well tolerated.

Objective: Our purpose was to determine the effectiveness of ranitidine in a 24-week, multicenter, double-blind, placebo-controlled, dose-comparing study of 201 patients with psoriasis.

Methods: Patients with moderate to severe psoriasis who had stopped systemic antipsoriatic therapy, including PUVA and UVB, for at least 10 weeks were included.

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The treatment of onychomycosis has previously often been protracted and unsuccessful. Terbinafine has been shown to be effective in short-term regimens. In this double-blind, placebo-controlled study, 148 patients with toenail dermatophytosis were randomized to treatment with either 250 mg terbinafine daily or placebo for 3 months.

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Squamous cell carcinomas were induced in hairless mice by repeated irradiations with UVB (280-320 nm, total dose 30 J/cm2) plus UVA (320-400 nm, total dose 168 J/cm2). The irradiated animals and non-irradiated controls were fed on diets with or without vitamin A supplementation (20,000 IU/kg). At the appearance of tumours, 30 to 43 weeks after the last irradiation, the vitamin A (retinol plus retinyl ester) concentrations in the serum, liver, epidermis and tumours and the retinol esterifying activities in microsomes from epidermis and tumours were measured.

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Skin biopsies from 5 patients with chronic plaque psoriasis were examined to test the effect of topical treatment with a new synthetic vitamin D3 analogue, MC 903, on epidermal interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF alpha). Skin biopsies, taken before therapy and after one and 2 weeks of therapy were examined immunohistologically. IL-6 was visualized using a partially purified polyclonal antiserum to crude supernatants of activated human blood monocytes before and after absorption with recombinant human IL-6.

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Biopsies from lesional and unaffected skin of 6 patients with psoriasis, taken before and during treatment with psoralen plus UVA (PUVA) were examined immunohistologically, using partially purified polyclonal antibodies to crude supernatants of activated human blood monocytes. By absorption with recombinant derived human monokines, we were able to demonstrate that interleukin-6 (IL-6) (but not IL-1 alpha or IL-1 beta) was located in a laminar and granular pattern in stratum corneum, and on epidermal cell membranes in the viable cellular epidermis. Before PUVA treatment, the intensity and the extension of staining for IL-6 were both markedly increased in lesional skin compared with uninvolved skin.

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In 10 in-patients with chronic plaque psoriasis, the antipsoriatic effect of MC903, a new synthetic analogue of vitamin D was evaluated. In each patient two symmetrical located psoriatic plaques were selected for the study. Topical treatment with MC903 cream (containing 1.

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Subcutaneous blood flow (SBF) was studied simultaneously in the upper arm at heart level and in the lower limb during positional changes and during leg exercise in seven healthy males. SBF was estimated by local clearance of 133Xenon registered by portable cadmium telluride detectors. Venous pressure was recorded directly on dorsum on the foot.

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Skin biopsies from five healthy subjects, taken before and after UVB irradiation, were examined using immunohistological techniques for the cytokines interleukin-I (IL-I) and tumour necrosis factor (TNF). Using polyclonal specific antibodies against IL-I and TNF, the two cytokines appeared identically located on the epidermal cell membranes of the stratum granulosum and stratum spinosum in unexposed skin. After UVB-exposure, the staining intensity for both IL-I/epidermal cell derived thymocyte-activating factor (ETAF) and TNF was markedly increased, and the epidermal staining included the basal cell layer.

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Skin blood flow (SBF) in allergic and irritant reactions was determined by laser Doppler flowmetry (LDF) in an attempt to differentiate these reactions in an objective way. 12 subjects with known allergy to nickel were patch tested with nickel sulphate, nickel chloride, and with sodium lauryl sulphate (SLS) 1, 2, 5 and 10%. A positive correlation between the concentration of SLS and SBF was found.

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To elucidate the effect of phototherapy on vitamin D metabolism in psoriatics, the serum concentrations of the major vitamin D metabolites (25-hydroxy-vitamin D (25(OH)D), 1,25-dihydroxy-vitamin D (1,25(OH)2D), and 24,25-dihydroxy-vitamin D (24,25(OH)2D)) were studied in 10 patients with disseminated psoriasis, both before and after phototherapy. Some 3-4 weeks of Goeckerman therapy induced significantly increased serum levels of 25(OH)D (mean: 24.6 ng/ml versus 54.

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The oedema formation of patch test reactions was quantified by high-frequency ultrasound measurement of skin thickness. Patch testing with nickel sulphate, nickel chloride and sodium lauryl sulphate 1, 2, 5 and 10% was performed in 12 individuals with known nickel allergy. Oedema was greater in allergic than in irritant reactions similar in strength according to the clinical reading.

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12 patients with known allergy to nickel were patch tested with nickel sulphate, nickel chloride, and sodium lauryl sulphate 1, 2, 5 and 10%. 2 parallel series were performed, i.e.

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The Langerhans' cell (LC) density is known to be reduced in skin lesions as compared to uninvolved skin in patients with psoriasis. It is, however, still unsettled whether the LC density in uninvolved psoriatic skin differs from the density in normal skin. We have enumerated epidermal LC in uninvolved skin from 15 patients with stable psoriasis and in 15 healthy subjects.

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To elucidate if psoriatic skin involvement induces changes in vitamin D metabolism, the serum concentrations of the major vitamin D metabolites (25-hydroxy-vitamin D(2+3) (25OHD), 1,25-dihydroxyvitamin D(2+3) (1,25(OH)2D), and 24,25-dihydroxyvitamin D(2+3) (24,25(OH)2D)) were studied in a group of patients with psoriasis, who had not been exposed to ultraviolet radiation at least three months before the investigation. Serum concentrations of 1,25(OH)2D were significantly reduced in 17 patients with disseminated psoriasis compared to healthy age and sex matched controls (22.3 pg/ml versus 35.

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Cutaneous and subcutaneous blood flow (CBF, SBF) were studied in non-lesional psoriatic skin (NLS) of 10 patients with only minimal psoriatic skin manifestations, using the local 133Xe washout method. Measurements of the CBF and SBF in the NLS of the patients and 10 normal individuals yielded no statistically significant differences. The results of the present study indicate that the activity of psoriasis can be monitored by the CBF measurements in the NLS, since previously published values for CBF of NLS have shown increasing values with increasing psoriatic activity.

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In 8 patients with psoriasis vulgaris, the cutaneous blood flow (CBF) was measured simultaneously in both involved and uninvolved psoriatic skin before (i.e., on the first day of hospitalization) and on the 3rd, 7th, 14th, and 28th days of treatment with tar.

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A bioavailability study was performed with a pill containing microcrystalline 8-MOP and a soft gelatine capsule containing dissolved 8-MOP in 35 patients receiving PUVA treatment. Peak plasma levels were almost doubled after ingestion of the capsule preparation in comparison with those recorded from the pill takers. The pharmaceutical formulation of 8-MOP preparations is of decisive importance for the bioavailability of the drug.

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Skin blood flow determined by laser Doppler flowmetry (LDF) and skin fold thickness (SFT) have been used to quantitate allergic contact dermatitis in the guinea pig maximization test (GPMT) using chlorocresol as the allergen. The closed patch test procedure itself influenced both LDF and SFT measurements when determined in 12 sham-treated guinea pigs. The LDF was maximal at 24 hours and the SFT at 48 hours.

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A patient with epilepsy and psoriasis in phenytoin therapy was treated with PUVA with no effect at all. The PUVA treatment failure was demonstrated as being due to abnormally low serum levels of 8-methoxypsoralen (8-MOP) during phenytoin therapy, while normal serum levels of 8-MOP were observed after phenytoin was discontinued. The effect is probably due to an induction of the hepatic enzyme system by phenytoin, leading to an increased metabolism of 8-MOP.

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