Early detection of oral bacteria causing gum infections and dental caries in children.

Background And Objectives: Periodontal diseases are resulted from gum infections and dental plaques, which are mainly caused by the bacterial agents. Since dental monitoring includes important prognostic roles, the aim of this study was to detect the most common periodontal pathogenic bacteria in children.

Materials And Methods: A total of 200 clinical samples were collected from dental plaques and gingival grooves.

Larvicidal Effects of Metabolites Extracted from and Species against the Forth Larval Stage of (Diptera: Culicidae).

Background: Larvicidal agents can be produced using microbial resources, which are environmentally friendly, biodegradable, and economical. The study's goal was to evaluate the larvicidal activity of metabolites isolated from (, and ) and () bacterial species against .

Methods: Four metabolites isolated from and strains were exanimated for larvicidal activity.

Transcriptional alteration of genes linked to gastritis concerning Helicobacter pylori infection status and its virulence factors.

Background: Helicobacter pylori infection and heterogeneity in its pathogenesis could describe diversity in the expression of inflammatory genes in the gastric tissue. We aimed to investigate transcriptional alteration of genes linked to gastritis concerning the H. pylori infection status and its virulence factors.

Evaluation of anti-biofilm potential of biosurfactant extracted from species.

Introduction: Bacterial natural products such as biosurfactants and surface-active agents are important compounds which exhibit many applications in the fields of medicine.

Aim: The aim of the present study was to isolate and identify Nocardia strains with high biosurfactant production and antibiofilm ability.

Materials And Methods: In the present study, a biosurfactant producing Nocardia species was isolated and identified by a laboratory method.

Variation of Housekeeping Genes in Clinical Isolates and Vaccine Strains of Bordetella pertussis.

Background: Bordetella pertussis causes serious contagious infections, primarily in childhood. A whole-cell vaccine, diphtheria-tetanus-whole cell pertussis (DTwP), has been used to protect against pertussis in children in Iran, but the pertussis cases have been increasing during recent years. We determined the allelic variation level of housekeeping genes in isolates recovered from pertussis patients and vaccine strains used in national vaccination program.

Dysbiosis of fecal microbiota and high frequency of spp., and Actinomycetes in patients with irritable bowel syndrome and gastroenteritis.

Aim: This study was aimed to characterize putative differences of fecal microbiota between irritable bowel syndrome (IBS) and gastroenteritis patients and healthy controls.

Background: New evidence proposed that gut microbiota has a deep effect on the balance between health and disease.

Patients And Methods: The presence of , spp.

Evaluation of New ELISA based on rLsa63 - rLipL32 antigens for serodiagnosis of Human Leptospirosis.

Background And Objectives: Timely diagnosis of leptospirosis is essential for an effective treatment. Large diversity of clinical symptoms has led leptospirosis diagnosis difficult. Researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are more reliable and available to public laboratories.

Nocardia isolation from clinical samples with the paraffin baiting technique.

Background: The genus Nocardia is a cause of infection in the lungs, skin, brain, cerebrospinal fluid, eyes, joints and kidneys. Nocardia isolation from polymicrobial specimens is difficult due to its slow growth. Several methods have been reported for Nocardia isolation from clinical samples.

Antibacterial effect of an herbal product persica on porphyromonas gingivalis and aggregatibacter actinomycetemcomitans: an in-vitro study.

Objective: The plant Salvadora persica is used for oral hygiene in many parts of the world. It has been suggested that it has antibacterial properties, in addition to its ability to mechanically remove plaques. The aim of this study was to assess the antimicrobial activity of the herbal product Persica containing Salvadora persica against periodontopathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in vitro.

Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients.

Background: Re-emergence of pertussis has been reported in Iran despite a high rate of vaccination coverage. Low efficacy of the vaccine might be due to the genetic divergence between clinical versus vaccine strains. In the current study, the genetic profiles of clinical isolates and vaccine strains of Bordetella pertussis (B.

DNA extraction from nocardia species for special genes analysis using PCR.

Background: Nocardia species have a complex cell wall structure similar to that of mycobacteria, and the extraction of DNA from this bacterium is extremely difficult. Currently, to identify Nocardia species particularly, it is essential to utilize molecular techniques.

Aims: In the present study, we investigated STET (sodium chloride-TRIS-EDTA-triton) buffer for the extraction of high-quality genomic DNA from 20 clinical and environmental isolates.

Diagnostic efficacy of lsa63 antigen for human leptospirosis.

Background: Timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. Leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the Leptospirosis diagnosis.

Pulmonary nocardiosis associated with cerebral abscess successfully treated by co-trimoxazole: a case report.

Nocardiosis is an acute or chronic infectious disease caused by the soil-borne filamentous bacteria belonging to the genus Nocardia. The organisms opportunistically infect both immunocompromised and immunocompetent individuals. The lungs are the primary site of infection and brain abscess is, by far, the most common complication following nocardial metastasis from pulmonary lesions.

Detection of Leptospires serogroups, Which Are Common Causes of Human Acute Leptospirosis in Guilan, Northern Iran.

Background: This study is performed to reveal most common species and subspecies of leptospires that are main causes of human leptospirosis in Guilan, Northern Province of Iran.

Methods: We performed IgM-ELISA and MAT on 282 blood samples from patients who attended to 3 hospitals in the flat area of Guilan Province with clinical symptoms consisted with leptospirosis. All specimens with titers ≥ 160 against at least one pathogenic strain in MAT and with titers ≥160 in IgM-ELISA were regarded confirmed positive cases indicative acute disease.

Molecular analysis of vanHAX element in vancomycin resistant enterococci isolated from hospitalized patients in Tehran.

Background: Vancomycin (glycopeptide)-resistant enterococci (VRE or GRE) can cause serious problems for hospitalized patients due to the limited options for treatment of VRE infections. As infection with VRE increases in hospitals, further knowledge about vancomycin resistant genes is needed.

Methods: Isolates of Enterococcus spp.