Effect of pH on solubility of white Mineral Trioxide Aggregate and Biodentine: An in vitro study.

The aim of this study was to evaluate the effect of acidic, neutral and alkaline environments on the solubility of white mineral trioxide aggregate (WMTA) and Biodentine (BD). Thirty-nine ring molds were randomly divided into three groups of A, B, and C (n = 12) with pH values of 7.4, 4.

A Stereomicroscopic Evaluation of Dentinal Cracks at Different Instrumentation Lengths by Using Different Rotary Files (ProTaper Universal, ProTaper Next, and HyFlex CM): An Ex Vivo Study.

Introduction. The aim of the present study was to evaluate the dentinal cracks after root canal preparation with rotary files: Gates Glidden, ProTaper Universal, ProTaper Next, and HyFlex CM at different instrumentation lengths. Methodology.

Production of recombinant antibodies using bacteriophages.

Recombinant antibody fragments such as Fab, scFv, diabodies, triabodies, single domain antibodies and minibodies have recently emerged as potential alternatives to monoclonal antibodies, which can be engineered using phage display technology. These antibodies match the strengths of conventionally produced monoclonal antibodies and offer advantages for the development of immunodiagnostic kits and assays. These fragments not only retain the specificity of the whole monoclonal antibodies but also easy to express and produce in prokaryotic expression system.

Recombinant human antibody fragment against tetanus toxoid produced by phage display.

Phage display technology is a powerful in vitro method for the identification of specific monoclonal antibodies (antibody fragments) to an antigenic target and allows the rapid generation and selection of high affinity, fully human antibodies directed toward any disease target appropriate for antibody therapy. In the present study, we exploited the phage display technology for the selection of an antigen binding fragment (Fabs) toward tetanus toxoid using human naïve phage antibody library constructed from peripheral blood lymphocytes of naïve human donors. The phages displaying Fab were subjected to three rounds of bio-panning with tetanus toxoid as antigen on a solid phase.

Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease.

Recombinant antibody fragments like single chain variable fragments (scFvs) represent an attractive yet powerful alternative to immunoglobulins and hold great potential in the development of clinical diagnostic/therapeutic reagents. Structurally, scFvs are the smallest antibody fragments capable of retaining the antigen-binding capacity of whole antibodies and are composed of an immunoglobulin (Ig) variable light (VL) and variable heavy (VH) chain joined by a flexible polypeptide linker. In the present study, we constructed a scFv against bovine IgA from a hybridoma cell line IL-A71 that secretes a monoclonal antibody against bovine IgA using recombinant DNA technology.

Immobilization of bile salt hydrolase enzyme on mesoporous SBA-15 for co-precipitation of cholesterol.

We describe herein a simple and effective strategy for immobilization of bile salt hydrolase enzyme by grafting glutaraldehyde groups inside channels of APTES functionalized SBA-15. The increase in glutaraldehyde concentration prevents leakage of enzyme but showed a steep decrease in enzyme activity in the immobilized matrix. So the degree of cross-linking should be the minimum possible to ensure sufficient stability without loss of activity.

The efficacy of neem extract on four microorganisms responsible for causing dental caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: an in vitro study.

Unlabelled: HISTORY AND OBJECTIVES: From the ancient time, neem used to be the traditional medicine for many diseases and was mainly used for cleaning the oral cavity. The incidence of dental caries was less a few decades ago but now the incidence of caries is very aggressive. This might be due to change in dietary habits, life style and more tendency toward processed food.

Sciatic nerve division: a cadaver study in the Indian population and review of the literature.

Introduction: The sciatic nerve is the largest nerve, with a long course in the inferior extremity. Its division into the tibial and common peroneal nerves can occur at any level from the sacral plexus to the inferior part of the popliteal space. These anatomical variations may contribute to clinical conditions such as piriformis syndrome, sciatica and coccygodynia.

Palmar dermatoglyphics in carcinoma breast of Indian women.

The present study was planned to assess the relationship of palmar dermatoglyphic patterns of hands in women with breast cancer and or at risk for developing breast cancer. This study was conducted on 100 histopathologically confirmed breast cancer patients in women and their digital dermatoglyphic patterns were studied to assess their association with the type and onset of breast cancer. Simultaneously 100 age-matched controls were also selected with no self or familial history of a diagnosed breast cancer and the observations were recorded.

Influence of silver nanoparticles on 2,3-bis(chloromethyl)anthracene-1,4,9,10-tetraone.

Size effect of silver nano particles on the photophysical properties of 2,3-bis(chloromethyl)anthracene-1,4,9,10-tetraone (BCMAT) have been investigated using optical absorption and fluorescence emission techniques. Silver NPs of different sizes have been prepared by two different methods. Quenching of fluorescence of BCMAT has been found to increase with decrease in the size of the silver NPs.

A cadaveric study in the Indian population of the brachialis muscle innervation by the radial nerve.

Radial nerve innervation to the brachialis muscle has been studied previously by different authors in Caucasian, Chinese, and Thai population. Present study was aimed to describe the radial nerve and musculocutaneous nerve contribution to the brachialis muscle and to elucidate racial differences between Indian and other populations. Hundred-forty superior extremities of 70 embalmed cadavers including 29 female and 41 male cadavers were dissected to study the innervation of brachialis muscle by musculocutaneous nerve and branch from the radial nerve.

Characterization of the smallest dimeric bile salt hydrolase from a thermophile Brevibacillus sp.

A thermophilic microorganism producing bile salt hydrolase was isolated from hot water springs, Pali, Maharashtra, India. This microorganism was identified as Brevibacillus sp. by 16S rDNA sequencing.

Brevibacillus sp: a novel thermophilic source for the production of bile salt hydrolase.

A thermophilic microorganism growing within the temperature range of 40-65 degrees C (optimum at 55 degrees C) was isolated from hot water springs near Konkan, Maharashtra, India. Based on 16S rDNA sequence analysis, it was concluded that the isolate belongs to the genus Brevibacillus. The present paper reports the isolation, identification, and standardization of fermentation conditions for the production of enzyme, bile salt hydrolase (EC 3.

Oxidative stress modulates membrane bound ATPases in brain regions of PCB (Aroclor 1254) exposed rats: protective role of alpha-tocopherol.

Polychlorinated biphenyls (PCBs) are a class of widely dispersed and environmentally persistent organic compounds. PCBs exhibit a wide range of toxicological effects including neurotoxicity. Vitamin E (alpha-tocopherol) is an important lipid soluble antioxidant placed in a special region of membranes.

Rapid in vitro screening of drug-metal ion interactions.

ABSTRACT The toxic side effects of synthetic drugs may, in part, be arising due to their interactions with essential metal ions, especially when the metal ions are administered along with the drug as mineral supplements. In this paper we report the feasibility of establishing such drug-metal ion interactions through in vitro spectrophotometric studies, which are rapid and can be used for routine screening prior to clinical studies. The interaction of the drugs levothyroxine and ranitidine with eight metal ions, copper(II), chromium(III), molybdenum(VI), magnesium(II), calcium(II), iron(II), manganese(II), and zinc(II), commonly used in mineral supplements, was verified through in vitro UV-visible spectrophotometric studies.