Phenotypic and genotypic detection of carbapenemase production among gram negative bacteria isolated from hospital acquired infections.

Objectives: To identify the carbapenemase producing Gram-negative bacteria (GNB) by phenotypic methods and to confirm the presence of resistant genes using real-time polymerase chain reaction (PCR).

Methods: This was a prospective study carried out at the Department of Microbiology, Sri Venkata Sai Medical College and Hospital, Mahabubnagar, India, from March 2018-2021. All samples were screened for carbapenem resistance by disc diffusion method and the VITEK2 compact system (bioMérieux, France).