Honeybee proteins in Saudi honeys: A shotgun gel-free proteomic study.

This study investigated the protein content of Acacia and Ziziphus honey samples from the southwestern region of Saudi Arabia following the shotgun gel-free proteomics. Honey proteins were extracted, digested by trypsin and the trypsin digests were separated and characterized using the LC-ESI-QTOF-MS (SCIEX X500R QTOF). The precursor masses of the trypsin digests were used to identify the proteins through searching the mascot spectral database search engine.