Publications by authors named "Spyridon P Deligiannis"
Purpose: Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian tissue follicle survival and transcriptomic signature requires further investigation. This study delves into the effects of vitrification on tissue morphology, function, and transcriptomic changes, helping to find possibilities for vitrification protocol improvements.
Methods: Ovarian cortex from 19 bovine animals were used to conduct pre- and post-vitrification culture followed by histological assessment, immunohistochemistry, and TUNEL assay.
Article Synopsis
- Pregnancy loss is frequently linked to chromosomal abnormalities, with a study revealing that about 50.4% of spontaneous losses had karyotypically abnormal products of conception, influenced by parental age.
- The researchers examined 94 cases of loss with normal karyotypes and discovered that 35.1% exhibited previously undetected chromosomal issues, raising the overall rate of abnormalities to 67.8%.
- Findings indicate that unlike viable pregnancies, where abnormalities are often limited to chorionic villi, pregnancy losses show more mosaicism in extra-embryonic tissues, highlighting the need for deeper analysis of genomic abnormalities to enhance understanding and treatment.
Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on a single-cell level or with limited RNA input. We describe different enzymes for reverse transcription and cDNA amplification, modified lysis buffer, and additional clean-up steps before cDNA amplification. We also detail an optimized single-cell RNA sequencing method for handpicked single cells, or tens to hundreds of cells, as input material to study mammalian preimplantation development.
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