Effect of the endothelin receptor antagonist bosentan on postischemic oxygen supply of the liver.

Endothelin evokes strong and longlasting constriction of postischemic sinusoids, leading to microcirculatory disturbances and local hypoxia, thereby causing liver damage. The aim of the study was to avoid the constrictive response of sinusoids by blocking endothelin receptors. In an in vivo ischemia-reperfusion model (21 female Wistar rats, 250-300 g) with portal decompression by a splenocaval shunt, hepatic ischemia was induced for 30 min by cross clamping of the hepatoduodenal ligament.

Effect of the endothelin receptor antagonist bosentan on postischemic liver microcirculation.

Unlabelled: One mechanism evoked by ischemia is endothelin mediated vasoconstriction of the hepatic vascular bed. Postischemic sinusoidal constriction leads to microcirculatory disturbances and reduced blood flow, thereby causing local hypoxia and liver damage. The aim of the study was to avoid the constrictive response of sinusoids by blocking endothelin receptors.

Resorbable ciprofloxacin/polyglycol acid carrier in the local therapy of chronic osteitis.

Polyglycolic test cylinders (PGA) of different sizes and with a different amount of Ciprofloxacin were synthezised and tested in vitro. The monofil 3,2×5 mm sized cylinders containing 3 mg of Ciprofloxacin showed the best release of the antibiotic up to half a year (measured by HPLC). In vivo setting: in the right femur of 66 rabbits osteomyelitis was induced by injection of 3×10(6) cfu of staph, aureus after the bone marrow had been sclerosed with sodium morrhuate 5%.

Reduction of hepatic reperfusion injury by indomethacin-mediated vasoconstriction: a rat model with temporary splenocaval shunt.

For reduction of radical formation during reperfusion, a lower oxygen supply by limiting the reperfusion flow rate should be beneficial for the organ. Thus, indomethacin was given prior to reperfusion for induction of temporary postischemic vasoconstriction. In an in vivo model (female Wistar rats, 200-250 g, n = 16) with portal decompression by a splenocaval shunt, hepatic ischemia was induced for 30 min by cross-clamping of the hepatoduodenal ligament followed by portal intravenous injection of indomethacin (2 mg/kg body wt) at the end of ischemia.

Penetration of ciprofloxacin into bone: a new bioabsorbable implant.

The implantation of antibiotic-containing cement beads became standard adjuvant local antibiotic therapy of chronic osteomyelitis. The new developed bioabsorbable drug carrier system of polyglycolic acid with the antibiotic Ciprofloxacin was tested in vitro and in vivo. The goal of this study was to determinate the penetration depth of Ciprofloxacin into bone cortex and marrow.

[Analysis of liver hemodynamics in severe ischemia and reperfusion injury after liver transplantation].

Aim: The pathogenesis of the ischemia-/reperfusion injury after liver transplantation is not fully understood. The purpose of this study was to analyse blood flow and vascular resistance of the hepatic artery and the portal vein after long ischemic time.

Methods: A model of orthotopic liver transplantation in pigs was used.

Liver transplantation in pigs: a model for studying reperfusion injury.

A model of orthotopic liver transplantation in pigs was developed to investigate reperfusion injury of the liver. To have direct access to the liver for organ monitoring a relaparotomy was performed 6 h after revascularization. The details of modification in anesthesia and surgical technique are given.

First isolation of Borrelia burgdorferi from an iris biopsy.

The persistence of Borrelia burgdorferi in six patients is described. Borrelia burgdorferi has been cultivated from iris biopsy, skin biopsy, and cerebrospinal fluid also after antibiotic therapy for Lyme borreliosis. Lyme Serology: IgG antibodies to B.

Predictors of smoking abstinence following a single-session restructuring intervention with self-hypnosis.

Objective: This study examined the relation of smoking and medical history, social support, and hypnotizability to outcome of a smoking cessation program.

Method: A consecutive series of 226 smokers referred for the smoking cessation program were treated with a single-session habit restructuring intervention involving self-hypnosis. They were then followed up for 2 years.

Monitoring of liver oxygenation during neuroleptanalgesia in the dog.

In an animal study (7 mongrel dogs) the effects of neuroleptanalgesia (NLA) and combinations of NLA with nitrous oxide (N2O) and isoflurane on the macro- and microcirculation of the liver were investigated. Measurements were made in three steps. After NLA alone the dogs were supplementarily ventilated with nitrous oxide/oxygen at a ratio of 2:1.

[Effect of cecostomy on the pathophysiology and prognosis of acute experimental pancreatitis].

In 76 male wistar rats with a median weight of 340 g acute pancreatitis was induced by injection of 2% sodium taurocholate into a temporarily closed duodenal loop. 40 animals received an additional cecostomy (group B), the others served as controls (group A). The postoperative figures for amylase, leucocyte count, and hemoglobin were nearly identical in both groups.

Neuropathology and pathogenesis of HIV encephalopathies.

The nervous system is frequently affected in patients with the acquired immune deficiency syndrome (AIDS). In addition to opportunistic CNS infections and cerebral lymphomas, approx. 20% of the patients develop HIV-associated encephalopathies.

Personality style and hypnotizability: the fix-flex continuum.

Since Mesmer, there has been much confusion about the inter-relationship between an individual's degree of hypnotizability, the personality style of the individual and the importance of the therapeutic strategy. Empirical and experimental research supports the hypotheses that there are: 1) biopsychosocial components of hypnotizability on a continuum ranging from ecologically insensitive (not modifiable by external stimuli) to ecologically sensitive (very modifiable by external stimuli); 2) biopsychosocial components that can be measured to identify an individual's degree of hypnotic capacity and responsivity; 3) distinct personality styles which correlate with low, mid-range and high hypnotizability on a fix (ecologically insensitive)-flex (ecologically sensitive) continuum; and 4) different clinical syndromes which correlate with these categorical distinctions. We propose that measuring hypnotizability and personality style is a way to clarify diagnosis and choose appropriate treatment strategies to maximize existing biopsychosocial resources of an individual with a specific problem in a particular context.

Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymphoid tissues facilitating infection of CD4+ T-helper cells.

Human immunodeficiency virus type 1 (HIV-1) infection causes progressive depletion of CD4/HIV-receptor-positive T helper lymphocytes, ultimately leading to AIDS. The major HIV reservoir and site of T-helper cell infection in lymphoid tissues, however, has remained poorly defined. The authors used in situ hybridization in combination with immunohistologic labeling techniques to identify the phenotype of HIV-infected cells in lymph nodes from patients at different stages of HIV-infection.

Distribution of human immunodeficiency virus (HIV) in the CNS of children with severe HIV encephalomyelopathy.

The presence and distribution of human immunodeficiency virus (HIV) were examined in the CNS of two children with severe HIV encephalitis and myelitis. Using polymerase chain reaction-mediated DNA amplification and subsequent Southern analysis, proviral HIV gag sequences were identified in brain tissue of both patients. In situ hybridization using antisense oligonucleotide probes revealed abundant HIV gag and env/nef RNAs selectively in areas with histopathological evidence for HIV-induced tissue damage.

[Sudden blindness in an AIDS patient. Simultaneous infection with cytomegalovirus and herpes simplex viruses and development of malignant non-Hodgkin lymphoma].

A 50 year old patient with non-diagnosed HIV-infection at onset of his illness developed impairment of vision and temporary double vision combined with severe hemianopsia. With normal fundus and suspicious Herpes simplex iritis therapy with Acyclovir was started. Primarily the patient showed a good response to the therapy and loss of vision could be prevented.

[Lymphoid tissues and AIDS: role of lymphocytes and follicular dendritic cells (FDC)].

Currently discussed models of AIDS pathogenesis attribute a pivotal role to HIV-variants developing in T cells during the course of the disease resulting in an increasingly rapid depletion of the infected T cells. Such models do not however explain the morphological findings observed in AIDS lymphadenopathy. To clarify the significance of the hyperplasia and subsequent destruction of the lymphoid follicles in HIV-related lymphadenopathy, we used in situ hybridization in combination with immunohistological labelling techniques to identify the phenotype of HIV-infected cells in lymph nodes.