Molecular and biological characterization of hepatitis B virus subgenotype F1b clusters: Unraveling its role in hepatocarcinogenesis.

Hepatitis B virus (HBV) subgenotype F1b infection has been associated with the early occurrence of hepatocellular carcinoma in chronically infected patients from Alaska and Peru. In Argentina, however, despite the high prevalence of subgenotype F1b infection, this relationship has not been described. To unravel the observed differences in the progression of the infection, an in-depth molecular and biological characterization of the subgenotype F1b was performed.

Unraveling the Relationship between Milk Yield and Quality at the Test Day with Rumination Time Recorded by a PLF Technology.

The study aimed to estimate the components of rumination time (RT) variability recorded by a neck collar sensor and the relationship between RT and milk composition. Milk test day (TD) and RT data were collected from 691 cows in three farms. Daily RT data of each animal were averaged for 3, 7, and 10 days preceding the TD date (RT).

Hepatitis B Virus X Gene Differentially Modulates Subgenotype F1b and F4 Replication.

Hepatitis B virus (HBV) is classified into ten genotypes and numerous subgenotypes (sgt). In particular, sgt F1b and sgt F4, native of Latin America, have been associated with differences in clinical and virological characteristics. Hepatitis B virus X protein (HBx) is a multifunctional regulatory protein associated with the modulation of viral transcription and replication.

Plasma estrone sulfate, clinical biochemistry, and milk yield of dairy cows carrying a fetus from a bull or its clone.

The aim of this article was to compare plasma estrone sulfate (E1SO4), clinical biochemistry, and milk yield of dairy cows carrying a female fetus from a bull (BULL) or from its clone (CLONE), evaluating also the relationship between the former variables and the birth weight of the newborn. Sixteen recipient dairy Friesian heifers (10 BULL and 7 CLONE) received a female embryo, obtained by in vitro embryo production and sexing by polymerase chain reaction with the semen of the BULL or the CLONE. Blood samples on all cows were obtained before feed distribution in the morning from jugular vein from 4 weeks before to 4 weeks after calving, to be analyzed for metabolic profile.

Body growth, hematological profile, and clinical biochemistry of heifer calves sired by a bull or its clone.

The aim of this paper was to compare body growth, hematological profile development, and clinical biochemistry in the female progeny of a sire with the female progeny of its clone. Sixteen Friesian female calves, 9 daughters from a tested bull (BULL) and 7 from its somatic cell nuclear transfer clone (CLONE) were monitored from birth to 60 wk of life. Body weight (BW), wither height (WH), hip height (HH), body length (BL), and hearth girth (HG) were measured at birth and 4, 8, 12, 16, 20, 24, 36, and 50 wk.

Evaluation of milk enzymes and electrolytes, plasma metabolites, and oxidative status in twin cows milked in an automatic milking system or twice daily in a conventional milking parlor.

The aim of this paper was to evaluate the effects of automatic milking (AM) on milk enzymes and minerals related to mammary epithelial integrity in comparison with twice-daily conventional milking (CM). One cow from each of 6 pairs of twins was assigned to be milked with AM or with CM throughout first lactation. Milk production was recorded and milk samples were collected at 4, 11, 18, 25, 32, and 39 wk of lactation (WOL) to determine fat and protein content, somatic cell count, pH, plasminogen (pl) and plasmin (Pl) activities, Na, K, and Cl.

Effect of automatic milking systems on milk yield in a hot environment.

A comparative study was performed to evaluate differences in milk yield between an automatic milking system (AMS) and a conventional herringbone milking parlor system. Two herds of Italian-Friesian cows were reared in the same barn, located in the Po Valley in northern Italy. Twenty-five primiparous cows and 10 multiparous cows were milked with an AMS, while at the same time 29 primiparous and 9 multiparous were milked twice daily in a milking parlor on the other side of the barn.

Welfare assessment based on metabolic and endocrine aspects in primiparous cows milked in a parlor or with an automatic milking system.

An automatic milking system (AMS) was compared with a traditional milking parlor (MP) to evaluate metabolic and psycho-physiological aspects of animal welfare. Twenty Italian Friesian heifers were allocated to 2 groups of 10 cows each after calving and maintained in the same free-stall barn. The first group was milked twice daily in a MP; the second group was milked in a single box AMS.

Effect of age at first calving on production traits and on difference between milk yield returns and rearing costs in Italian Holsteins.

The 305-d yield records of 1,048,942 Italian Holstein-Friesian heifers were used to determine the effect of age at first calving on milk yield and fat and protein concentration. Research showed that there is a positive effect of age at first calving on milk yield and fat percentage and a negative effect on protein percentage. In six scenarios of milk price and rearing costs (2 x 3, respectively), the effect of age at first calving on the difference between milk yield returns and rearing costs was calculated.