Publications by authors named "Soyeon Yang"
Introduction: Cracked teeth present diagnostic and treatment challenges due to their complex etiology and uncertain prognoses. This study evaluated the potential of Quantitative Light-induced Fluorescence (QLF) technology for diagnosing cracked teeth and its utility in predicting the need for root canal treatment (RCT).
Methods: A total of 207 cracked teeth from 149 patients diagnosed between April 2019 and April 2023 were analyzed.
We investigated the effectiveness of exome sequencing (ES) in diagnosing ethnically diverse patients with rare genetic disorders. A total of 18,994 patients referred to a single reference laboratory for ES between 2020 and 2022 were studied for the diagnostic rate and factors influencing the diagnostic rate. The overall diagnostic rate was 31.
View Article and Find Full Text PDFGenome editing using CRISPR-Cas9 nucleases is based on the repair of the DNA double-strand break (DSB). In eukaryotic cells, DSBs are rejoined through homology-directed repair (HDR), non-homologous end joining (NHEJ) or microhomology-mediated end joining (MMEJ) pathways. Among these, it is thought that the NHEJ pathway is dominant and occurs throughout a cell cycle.
View Article and Find Full Text PDFCRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells.
Comput Struct Biotechnol J
June 2020
Article Synopsis
- CRISPR-Cas9 allows targeted DNA editing by creating breaks in DNA, which are repaired through processes like non-homologous end joining (NHEJ), leading to insertions and deletions but rarely substitutions.
- A new method called CRISPR-Sub was developed to accurately detect substitutions in sequencing data by comparing samples treated with CRISPR to control samples, identifying 'hotspot positions' where substitutions are more frequent.
- Research showed that apparent substitution frequencies were around 0.8% in HeLa cells, predominantly near CRISPR-Cas9 cleavage sites, and that these substitutions significantly decreased in cell lines with inhibited NHEJ, indicating that NHEJ contributes to these DNA changes.