Publications by authors named "Sowmya Kalaga"
Iodine-contrast micro-computed tomography (microCT) 3D imaging provides a non-destructive and high-throughput platform for studying mouse embryo and neonate development. Here we provide protocols on preparing mouse embryos and neonates between embryonic day 8.5 (E8.
View Article and Find Full Text PDFArticle Synopsis
- The text refers to a correction made to a previously published article with the DOI: 10.1038/s42003-018-0226-0.
- The correction is likely important for ensuring the accuracy and integrity of the research findings presented in the original article.
- Readers are encouraged to check the corrected version for updated information that may affect the conclusions or interpretations of the study.
Article Synopsis
- Advances in next generation sequencing have made it easier to study genetics, but understanding genetic causes of eye diseases is still tough due to cost and limited access to human genetic data.
- The International Mouse Phenotyping Consortium conducted a study evaluating 4,364 genes and found that 347 of them affect eye traits, with 75% being previously unknown in eye disease research.
- This significant increase in known genes related to eye conditions could have future implications for understanding eye development and diseases in humans.
Retinal function relies on precisely organized neurons and synapses and a properly patterned vasculature to support them. Alterations in these features can result in vision loss. However, our understanding of retinal organization pathways remains incomplete because of a lack of methods to rapidly identify neuron and vasculature regulators in mammals.
View Article and Find Full Text PDFIn this work, we report the use of iodine-contrast microCT to perform high-throughput 3D morphological analysis of mouse embryos and neonates between embryonic day 8.5 to postnatal day 3, with high spatial resolution up to 3µm/voxel. We show that mouse embryos at early stages can be imaged either within extra embryonic tissues such as the yolk sac or the decidua without physically disturbing the embryos.
View Article and Find Full Text PDFApproximately one-third of all mammalian genes are essential for life. Phenotypes resulting from knockouts of these genes in mice have provided tremendous insight into gene function and congenital disorders. As part of the International Mouse Phenotyping Consortium effort to generate and phenotypically characterize 5,000 knockout mouse lines, here we identify 410 lethal genes during the production of the first 1,751 unique gene knockouts.
View Article and Find Full Text PDF