Decoding transaminase motifs: Tracing the unknown patterns for enhancing the accuracy of computational screening methodologies.

Transaminases, enzymes known for their amino group transfer capabilities, encompass four distinct subfamilies: D-alanine transaminase (DATA), L-selective Branched chain aminotransferase (BCAT), and 4-amino-4-deoxychorismate lyase (ADCL) and R-selective aminotransferase (RATA). RATA enzymes are particularly valuable in biocatalysis for synthesizing chiral amines and resolving racemic mixtures, yet their identification in sequence databases is challenging due to the lack of robust motif-based screening methods. Constructing a sequence dataset of transaminases, and categorizing them to various subfamilies, the conserved motifs are screened over the experimentally known ones, and the novel motifs are explored.

Environmental pollution mitigation through utilization of carbon dioxide by microalgae.

Anthropogenic emissions of CO have reached a critical level and the global surface temperature is expected to rise by 1.5 °C between 2030 and 2050. To ameliorate the current global warming scenario, the research community has been struggling to find more economical and innovative solutions for carbon sequestration.

Significance of Convection and Internal Heat Generation on the Thermal Distribution of a Porous Dovetail Fin with Radiative Heat Transfer by Spectral Collocation Method.

A variety of methodologies have been used to explore heat transport enhancement, and the fin approach to inspect heat transfer characteristics is one such effective method. In a broad range of industrial applications, including heat exchangers and microchannel heat sinks, fins are often employed to improve heat transfer. Encouraged by this feature, the present research is concerned with the temperature distribution caused by convective and radiative mechanisms in an internal heat-generating porous longitudinal dovetail fin (DF).

Assessment of thermal distribution through an inclined radiative-convective porous fin of concave profile using generalized residual power series method (GRPSM).

The thermal distribution in a convective-radiative concave porous fin appended to an inclined surface has been examined in this research. The equation governing the temperature and heat variation in fin with internal heat generation is transformed using non-dimensional variables, and the resulting partial differential equation (PDE) is tackled using an analytical scheme, generalized residual power series method (GRPSM). Moreover, a graphical discussion is provided to examine the consequence of diverse non-dimensional variables including the parameters of convection-conduction, ambient temperature, radiation, heat generation, and porosity effect on the thermal field of the fin.

Flow of hybrid nanofluid across a permeable longitudinal moving fin along with thermal radiation and natural convection.

Background: The numerical investigation of nanoparticles embedded water based hybrid nanoliquid flow over porous longitudinal fin moving with constant velocity is carried out together with thermal radiation and natural convection condition. Darcy's model is implemented for the flow behaviour. The two types of boundary conditions are considered at the tip i.

Analysis of silver binding nucleolar organizer regions in exfoliative cytology smears of potentially malignant and malignant oral lesions.

Nucleolar organizer regions are nucleolar components that contain proteins that are stained selectively by silver methods; they can be identified as black dots throughout the nucleolus and are known as silver binding nucleolar organizer regions (AgNOR). The number of AgNOR is related to the cell cycle and the proliferative activity of the cells. We investigated AgNOR using exfoliative cytology smears of potentially malignant oral lesions.

A case of unilateral atypical orofacial pain with Eagle's syndrome.

Eagle's syndrome is not an uncommon condition, but less known to physicians, where an elongated styloid process or calcified stylohyoid ligament compresses the adjacent anatomical structures leading to orofacial pain. Diagnosis is made with appropriate radiological examination. Nonsurgical treatment options include reassurance, analgesia, and anti.

Prevalence and characterization of opportunistic candidal infections among patients with pulmonary tuberculosis.

Background: Although Candida albicans remains the most common cause of human candidiasis, the frequency of infection attributed to other members of the genus is also increasing. Hence, the present study was carried out to know the prevalence of opportunistic candidal infection in tuberculosis, and if positive, the species of Candida that is most commonly associated.

Materials And Methods: The present study comprised sixty pulmonary tuberculosis patients who were divided into (1) fresh or untreated group, (2A) chronic or treated group having no complications and (2B) having complications, comprising twenty patients each, respectively.

Discrete structural features among interface residue-level classes.

Background: Protein-protein interaction (PPI) is essential for molecular functions in biological cells. Investigation on protein interfaces of known complexes is an important step towards deciphering the driving forces of PPIs. Each PPI complex is specific, sensitive and selective to binding.

Linking structural features of protein complexes and biological function.

Protein-protein interaction (PPI) establishes the central basis for complex cellular networks in a biological cell. Association of proteins with other proteins occurs at varying affinities, yet with a high degree of specificity. PPIs lead to diverse functionality such as catalysis, regulation, signaling, immunity, and inhibition, playing a crucial role in functional genomics.

Touch imprint cytology: a rapid diagnostic tool for oral squamous cell carcinoma.

Techniques for intraoperative pathologic examination of oral squamous cell carcinoma are rare in the literature. We evaluated the advantages and limitations of touch imprint cytology for intraoperative diagnosis of oral squamous cell carcinoma. We used 30 incisional biopsies of clinically diagnosed oral squamous cell carcinoma and compared touch imprint cytology to histopathological sections.

Oral focal mucinosis: a rare case with literature review.

Oral focal mucinosis (OFM) is an unusual disease that affects diverse localised areas of the mouth, where the connective tissue undergoes focal degeneration. It was described for the first time by Tomich in 1974. It presents as an asymptomatic pedunculated or sessile growth, commonly on the gingiva, with most cases being in women.

Prevalence, patterns, and factors associated with bleeding tendencies in dengue.

Introduction: The pattern of bleeding tendencies in dengue and its corellation with platelet count and other factors requires clarification.

Methodology: A retrospective study on bleeding tendencies in adults with dengue and platelet counts of less than 100,000 per mm(3) was conducted. Factors associated with bleeding were analyzed.

Oral Crohn's disease.

'Crohn's disease' is an inflammatory granulomatous disease of the gastrointestinal tract with extra-intestinal manifestations. Oral lesions may precede the intestinal disease and serve as a source for histological diagnosis. We present a case of orofacial Crohn's disease where orofacial symptoms were present for about 13 years and occasional constipation was present, since 6 months.

Characterization of the interaction between heterodimeric αvβ6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics.

Urokinase plasminogen activator receptor (uPAR) and the epithelial integrin αvβ6 are thought to individually play critical roles in cancer metastasis. These observations have been highlighted by the recent discovery (by proteomics) of an interaction between these two molecules, which are also both implicated in the epithelial-mesenchymal transition (EMT) that facilitates escape of cells from tissue barriers and is a common signature of cancer metastases. In this study, orthogonal in cellulo and in vitro functional proteomic approaches were used to better characterize the uPAR·αvβ6 interaction.

Management of Oro-Nasal Fistula Using Andrew's Bridge: A Case Report.

Oro-nasal fistula is the most common complication following the surgical closure of the cleft palate. Retention is the paramount factor in the successful prosthodontic habilitation of cleft palate patients. Various precision attachments have provided us with the opportunity to make the prosthesis fixed removable type; giving a double advantage to the patient i.

Collagenase-3 expression in periapical lesions: an immunohistochemical study.

Collagenase-3 (matrix metalloproteinase-13) is a metalloproteinase (MMP) that is associated with bone lesions and exhibits variable expression patterns in odontogenic cysts; it may play a role in regulating focal proliferation and maturation of jaw cyst epithelium. We studied the localization, staining intensity and distribution of collagenase-3 in 13 periapical granulomas with epithelium, 16 periapical granulomas without epithelium and 10 radicular cysts using archived formalin fixed, paraffin embedded tissues. A monoclonal antibody against human collagenase-3 was used to evaluate its expression.

Necrotizing soft-tissue infection: laboratory risk indicator for necrotizing soft tissue infections score.

Necrotizing soft tissue infections (NSTI) can be rapidly progressive and polymicrobial in etiology. Establishing the element of necrotizing infection poses a clinical challenge. A 64-year-old diabetic patient presented to our hospital with a gangrenous patch on anterior abdominal wall, which progressed to an extensive necrotizing lesion within 1 week.

A site for direct integrin αvβ6·uPAR interaction from structural modelling and docking.

Integrin αvβ6 is an epithelially-restricted heterodimeric transmembrane glycoprotein, known to interact with the urokinase plasminogen activating receptor (uPAR), playing a critical role in cancer progression. While the X-ray crystallographic structures of segments of other integrin heterodimers are known, there is no structural information for the complete αvβ6 integrin to assess its direct interaction with uPAR. We have performed structural analysis of αvβ6·uPAR interactions using model data with docking simulations to pinpoint their interface, in accord with earlier reports of the β-propeller region of integrin α-chain interacting with uPAR.

Protein-protein interactions and prediction: a comprehensive overview.

Molecular function in cellular processes is governed by protein-protein interactions (PPIs) within biological networks. Selective yet specific association of these protein partners contributes to diverse functionality such as catalysis, regulation, assembly, immunity, and inhibition in a cell. Therefore, understanding the principles of protein-protein association has been of immense interest for several decades.

Chrysomya bezziana oral myiasis.

Myiasis is an opportunistic infestation of human and vertebrate animals with dipterous larvae. Oral myiasis is a rare condition associated with poor oral hygiene, mental disability, halitosis and other conditions. We present a case report of an adult mentally challenged woman with extensive necrotic oral lesion burrowing into the hard palate through which three live maggots (larvae) were seen emerging out.

Insights from the structural analysis of protein heterodimer interfaces.

Protein heterodimer complexes are often involved in catalysis, regulation, assembly, immunity and inhibition. This involves the formation of stable interfaces between the interacting partners. Hence, it is of interest to describe heterodimer interfaces using known structural complexes.