Publications by authors named "Sousa G"
This study aims to shed light on the mechanism and kinetics of 1,4-dioxane degradation by hydroxyl radical (OH) across various solvation conditions to evaluate electronic and structural properties at the MP2/aug-cc-pVTZ level. Transition states (TS) structures determined in the gas phase and SMD solvation model reveal similar hydrogen abstraction patterns. In contrast, the explicit solvation model (ES) introduces significant changes, suggesting a kinetic preference for axial pathways.
View Article and Find Full Text PDFBackground: The 3D printing of macro- and mesoporous biomimetic grafts composed of polycaprolactone (PCL) infused with nanosized synthetic smectic clay is a promising innovation in biomaterials for bone tissue engineering (BTE). The main challenge lies in achieving a uniform distribution of nanoceramics across low to high concentrations within the polymer matrix while preserving mechanical properties and biological performance essential for successful osseointegration.
Methods: This study utilized 3D printing to fabricate PCL scaffolds enriched with nanosized synthetic smectic clay (LAP) to evaluate its effects on structural, chemical, thermal, mechanical, and degradative properties, with a focus on in vitro biological performance and non-toxicity.
Background: , a notable plant species, has garnered interest for its medicinal properties, including anti-inflammatory, antibacterial and antiviral effects. A vaccine for Chikungunia virus is still under evaluation and no specific antiviral drug has been licensed to date.
Objective: The work investigated antiviral activity of ethyl acetate (EAEF) and methanolic (EMF) extracts from leaves in mammalian cells exposed to (CHIKV).
Article Synopsis
- Pain management for thoracic trauma patients traditionally relies on opioids, which can have significant side effects; locoregional anesthesia/analgesia (LRAA) offers a targeted alternative.
- The study analyzed 43 LRAA procedures in 33 ICU patients and found that 50% who received LRAA avoided intubation, leading to shorter ICU stays (9 vs. 13 days) and no severe complications from the LRAA techniques.
- Results indicate that early use of LRAA can enhance clinical outcomes for thoracic trauma patients, highlighting its potential but also the need for further investigation to understand its full benefits.
A single 1.7 cm × 1.2 cm × 1 cm focal, raised, smooth, round, pink to flesh-colored mass protruding from the chorioallantois of the zonary placenta was found during Cesarean section in a primiparous pug bitch.
View Article and Find Full Text PDFOsteoprotegerin and Vascular Dysfunction in Patients with Stage 3 Chronic Kidney Disease and Those without Renal Dysfunction: A Case-Control Study.
Arq Bras Cardiol
October 2024
Background: Osteoprotegerin (OPG) is a marker of vascular calcification and cardiovascular risk in patients with chronic kidney disease (CKD).
Objective: This study aimed to compare and correlate OPG values with flow-mediated dilation (FMD) and pulse wave velocity (PWV) measurements in patients in stage 3 CKD and those without renal dysfunction.
Methods: This case-control study was conducted in a specialized hypertension center in 2022.
Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes' expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients' samples.
View Article and Find Full Text PDFThe global prevalence of counterfeit and low-quality pharmaceuticals poses significant health risks and challenges in medical treatments, creating a need for rapid and reliable drug screening technologies. This study introduces a cost-effective electrochemical paper-based device (ePAD) modified with functionalized bamboo-derived biochar (BCF) for the detection of paracetamol in substandard medicines. The sensor was fabricated using a custom 3D-printed stencil in PLA, designed for efficient production, and a 60:40 (m/m) graphite (GR) and glass varnish (GV) conductive ink, resulting in a robust and sensitive platform.
View Article and Find Full Text PDFEmerging evidence suggests that aberrant expression levels of Sigma1 (, also known as sigma-1 receptor) have been implicated in the progression of various diseases, including cancer. However, its significance in oral cancer (OC) has not been thoroughly explored. To advance in this field, the present study aimed to investigate the impact of knockdown in oral cancer cells.
View Article and Find Full Text PDFArticle Synopsis
- Drug-induced interstitial lung disease (DI-ILD) is a rising complication associated with certain anti-cancer therapies, particularly newer drugs like trastuzumab-deruxtecan, affecting their safe use.
- Different drugs cause lung damage via various mechanisms—primarily cytotoxic and immune-mediated—but the exact pathways remain unclear.
- Effective diagnosis and management of DI-ILD require teamwork among healthcare professionals, patient education for early detection, and further research to develop standard treatment protocols.
Article Synopsis
- - Obesity requires complex preoperative assessments for bariatric surgery, and this study aims to evaluate a new protocol for pre-anesthesia consultations to improve efficiency and reduce costs.
- - A retrospective analysis of 206 cases was conducted using various statistical tests to compare consultations conducted with and without the proposed protocol.
- - Results indicated that the protocol significantly reduced costs and consultation duration, but did not impact surgery delays or hospital stay lengths, suggesting it effectively optimizes management in bariatric surgery.
Article Synopsis
- Two new caffeoyl triterpenes, -lupacosmeol (1) and a mixture of -lupacosmeol (2), were isolated from plant roots along with two known compounds.
- Their structures were analyzed using advanced chemical techniques like 1D and 2D NMR, IR spectroscopy, and HRESIMS.
- The mixture 2 was found to be cytotoxic against multiple cancer cell lines, especially inhibiting the growth of SK-MEL-28, with its activity linked to the interaction between the two triterpenes in the mixture.
Article Synopsis
- - The study analyzed 64 patients with small cell lung cancer (SCLC) to assess the expression of Delta-like ligand 3 (DLL3) and other neuroendocrine markers using immunohistochemistry and digital pathology tools; DLL3 was found expressed in 72.3% of patients.
- - The majority of patients were older adults (median age over 71), and notable findings included high expression rates of ASCL1 (97%) and a potential new role for TTF-1 as a biomarker, as it was positive in half of the cases.
- - The research highlights the variability of neuroendocrine marker expression in SCLC, suggesting that DLL3 could be a therapeutic target, while the
Article Synopsis
- This study looks at how 16 managers from different parts of Brazil feel about the difficulties in caring for older adults who need help in their local health services.
- It finds that families often struggle due to lack of training and support, and there aren't enough health professionals to help, creating a big problem.
- The research suggests that to improve care, we need more trained workers and better public support to help families take care of older people in a way that meets their needs.
Article Synopsis
- Access to safe surgery is a human right, but significant disparities exist between high-income and low-to-middle-income countries in terms of surgical care.* -
- The LASOS-Peds study is a 14-day international research project exploring the rates of complications after pediatric surgeries in Latin America, focusing on both elective and emergency cases.* -
- Approved by an Institutional Review Board, the findings will be published in peer-reviewed journals and shared at international conferences, with the aim of improving pediatric surgical outcomes.*
This randomized controlled trial investigated the effectiveness of an affect-adjusted, supervised, multimodal, online, and home-based exercise group protocol as an adjunct therapy to antidepressants on depressive symptoms, cardiorespiratory fitness, and side effects related to antidepressants in adults with major depression (MDD, diagnosed by a clinician). Depressive symptom scales were administered by a psychiatrist and self-reported. A health-related measure (i.
View Article and Find Full Text PDFThis article aims to analyze spatial and temporal patterns of maternal mortality in Brazil during the period 2010-2020 and identify related socioeconomic indicators. We conducted an ecological study of the maternal mortality ratio (MMR) in Brazil's municipalities using secondary data. Temporal analysis was performed using the joinpoint method.
View Article and Find Full Text PDFP53 Phase separation is crucial towards amyloid aggregation and p63 and p73 have enhanced expression in tumors. This study examines the phase behaviors of p53, p63, and p73. Here we show that unlike the DNA-binding domain of p53 (p53C), the p63C and p73C undergo phase separation, but do not form amyloids under physiological temperatures.
View Article and Find Full Text PDFSelenium (Se) is a nutrient whose daily intake is often below the recommended levels in people. Biofortification with Se is a method to increase this intake by raising the Se concentration in tomato fruits, an effect dependent on sources and modes of application. Additionally, Se application can promote the enhancement of other compounds in tomato fruits, altering their metabolism, which may increase the fruit's shelf life.
View Article and Find Full Text PDFArticle Synopsis
- The Herpotrichiellaceae family consists of pathogenic fungi linked to diseases like chromoblastomycosis and phaeohyphomycosis, which present complex clinical symptoms and are found globally.
- Identifying these fungi is difficult due to subjective morphological methods, leading researchers to rely on molecular techniques, particularly genetic sequencing of ribosomal DNA.
- This study developed optimized PCR-Multiplex methodologies to improve the identification of these fungi, aiming to enhance infection management and provide more accurate epidemiological data.
The search for less harmful, ecologically efficient, more specific, and natural alternatives for the control of pathogens is essential. Bauhinia variegata lectin (BvL) is a protein that has numerous biological activities, including antifungal. The present study examines the potential in vitro of B.
View Article and Find Full Text PDFObjective: to map the scientific literature on the validity of instruments for evaluating the quality of services provided in primary health care for chronic diseases related to systemic arterial hypertension, diabetes mellitus, leprosy and tuberculosis.
Method: scoping review, following the Joanna Briggs Institute method and described in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. 13 databases and gray literature were included.
Shotgun proteomics analysis presents multifaceted challenges, demanding diverse tool integration for insights. Addressing this complexity, OmicScope emerges as an innovative solution for quantitative proteomics data analysis. Engineered to handle various data formats, it performs data pre-processing - including joining replicates, normalization, data imputation - and conducts differential proteomics analysis for both static and longitudinal experimental designs.
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