Publications by authors named "Soumya Ranjan Mahapatra"

Article Synopsis
  • Enterovirus 71 is a virus that causes hand-foot and mouth disease, particularly in children, and currently there is no approved vaccine due to the virus's evolving genome.
  • Researchers have developed a new vaccine using T-cell epitopes from the virus and added an adjuvant to enhance immune response.
  • The vaccine's design shows promising immunogenicity, safety for human use, and effectiveness in stimulating a strong immune response according to computational analysis.
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The bacterial secretome represents a comprehensive catalog of proteins released extracellularly that have multiple important roles in virulence and intercellular communication. This study aimed to characterize the secretome of an environmental isolate Pseudomonas aeruginosa S-8 by analyzing trypsin-digested culture supernatant proteins using nano-LC-MS/MS tool. Using a combined approach of bioinformatics and mass spectrometry, 1088 proteins in the secretome were analyzed by PREDLIPO, SecretomeP 2.

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Klebsiella pneumoniae, which is among the top three pathogens on WHO's priority list, is one of the gram-negative bacteria that doctors and researchers around the world have fought for decades. Capsular polysaccharide (CPS) protein is extensively recognized as an important K. pneumoniae virulence factor.

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The extremely widespread and ubiquitous nature of plastics, estimated to boost its global production by 26 billion tons till 2050. The large chunks of plastic waste that decomposed down to micro- or nano plastics (MNPs) leads to various ill effects on biological entities. The conventional PET detection methods lack rapid detection of microplastics due to variances in microplastic features, long-drawn-out sample pre-processing procedures and complex instrumentation.

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Acinetobacter baumannii is one of the major pathogenic ESKAPE bacterium, which is responsible for about more than 722,000 cases in a year, globally. Despite the alarming increase in multidrug resistance, a safe and effective vaccine for Acinetobacter infections is still not available. Hence in the current study, a multiepitope vaccine construct was developed using linear B cell, cytotoxic T cell, and helper T cell epitopes from the antigenic and well-conserved lipopolysaccharide assembly proteins employing systematic immunoinformatics and structural vaccinology strategies.

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is a human bacterial pathogen that can cause a wide range of symptoms. As virulent and multi-drug-resistant strains of have evolved, invasive infections in hospitals and the community have become one of the leading causes of mortality and morbidity. The development of novel techniques is therefore necessary to overcome this bacterial infection.

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Staphylococcus aureus has been widely reported to be majorly responsible for causing nosocomial infections worldwide. Due to an increase in antibiotic-resistant strains, the development of an effective vaccine against the bacteria is the most viable alternative. Therefore, in the current work, an effort has been undertaken to develop a novel peptide-based vaccine construct against S aureus that can potentially evoke the B and T cell immune responses.

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COVID-19 is a systemic disease affecting multiple organ systems and caused by infection with the SARS-CoV-2 virus. Two years into the COVID-19 pandemic and after the introduction of several vaccines, the pandemic continues to evolve in part owing to global inequities in access to preventive and therapeutic measures. We are also witnessing the introduction of antivirals against COVID-19.

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Article Synopsis
  • - Acinetobacter baumannii is a dangerous pathogen linked to severe infections and high mortality rates, leading researchers to investigate its pilus assembly protein, FilF, for vaccine development.
  • - Using bioinformatics, the study identified multiple B and T cell epitopes, narrowing it down to fourteen promising non-allergenic and non-toxic peptides for a multi-epitope vaccine.
  • - The vaccine was designed with specific linkers and adjuvants and demonstrated stable interaction with the immune receptor TLR-4, paving the way for future experimental approaches to combat this bacterium.
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  • Enterococcus faecium is a dangerous bacterium with no current treatments or vaccines, prompting research into a new vaccine design.
  • The study focused on a specific protein, PBP 5, to identify key epitopes for immune responses, ultimately selecting multiple B and T cell epitopes for a novel chimeric vaccine.
  • Preliminary results show the developed vaccine is stable, effective, and capable of triggering a strong immune response, paving the way for future experimental trials.
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Abstract: , an ESKAPE pathogen causes many fatal clinical diseases in humans across the globe. Despite an increase in clinical instances of Pseudomonas infection, there is currently no effective vaccine or treatment available. The major membrane protein candidate of the bacterial cell is known to be a critical component for cellular bacterial susceptibility to antimicrobial peptides and survival inside the host organisms.

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Article Synopsis
  • K. pneumoniae is an emerging pathogen that causes serious health issues, particularly in newborns, and poses a significant threat in healthcare settings due to its high fatality rates and virulence linked to its capsular polysaccharide.
  • The study utilized immunoinformatics to create a multi-peptide vaccine composed of various immune epitope types, selected based on several criteria to ensure effectiveness and compatibility with the human immune system.
  • Results indicated that the designed vaccine showed strong potential for inducing immune responses and efficient expression in a laboratory setting, suggesting it could be a promising candidate for combating K. pneumoniae in future research.
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  • *This research explores key genes and enzymes involved in carotenoid production across 22 different microalgae species using bioinformatics and comparative genomics.
  • *A total of 403 enzymes were identified, with 85 being uncharacterized, providing insights that could lead to the development of genetically modified algae for higher carotenoid content.
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is one of the most notorious Gram-positive bacteria with a very high mortality rate. The WHO has listed as one of the ESKAPE pathogens requiring urgent research and development efforts to fight against it. Yet there is a major layback in the advancement of effective vaccines against this multidrug-resistant pathogen.

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Pneumonia is a major endemic disease around the world, and an effective vaccine is the need of the hour to fight against the disease. When there are no appropriate antiviral and associated therapies available, vaccine development becomes even more essential. Therefore, in the present study, a variety of immunoinformatics techniques was utilized to develop a novel multi-epitope vaccine that targets the highly immunodominant type 3 fimbrial protein of Klebsiella pneumoniae, the causal organism for pneumonia.

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Article Synopsis
  • Staphylococcus aureus infections are a rising global health threat due to their severe impact and increasing resistance to antibiotics, prompting WHO to classify them as a high priority pathogen.
  • The study aims to develop a multi-epitope vaccine by identifying specific antigenic peptides from the bacteria's surface proteins, ClfA and ClfB, which offer strong immune responses and compatibility with immune receptor molecules.
  • Advanced techniques like molecular modeling and simulations confirmed the stability of the vaccine structure, while codon optimization ensures effective expression, making this vaccine candidate promising against multi-drug-resistant strains.
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Article Synopsis
  • * A new multi-epitope subunit vaccine was developed using an immunoinformatic approach, featuring conserved epitopes from various multidrug resistance protein families that may offer cross-protection.
  • * The vaccine candidate showed promising properties like high immunogenicity and strong binding affinity to HLA alleles, indicating potential for effective T-cell and B-cell immune responses against typhoidal Salmonella serovars.
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Since the emergence of SARS-CoV-1 (2002), novel coronaviruses have emerged periodically like the MERS- CoV (2012) and now, the SARS-CoV-2 outbreak which has posed a global threat to public health. Although, this is the third zoonotic coronavirus breakout within the last two decades, there are only a few platforms that provide information about coronavirus genomes. None of them is specific for the virulence glycoproteins and complete sequence-structural features of these virulence factors across the betacoronavirus family including SARS-CoV-2 strains are lacking.

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Article Synopsis
  • - A study explores the development of a potential vaccine for SARS-CoV-2, the virus behind the COVID-19 pandemic, focusing on mapping epitopic sequences from crucial glycoproteins, particularly the spike protein.
  • - Researchers created a multi-epitopic vaccine model that includes various immune response elements and found new epitopes that are predicted to be highly effective, safe, and applicable for a large portion of the global population.
  • - The study successfully developed a 3D structure of the vaccine and conducted simulations showing stable interactions between the vaccine and immune receptors, indicating promising potential for immune response.
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In light of increasing algal genomics data and knowledge of biosynthetic pathways responsible for biofuel production, an integrated resource for easy access to all information is essential to improve our understanding of algal lipid metabolism. Against this backdrop, dEMBF v2.0, a significantly updated and improved version of our database of microalgae lipid biosynthetic enzymes for biofuel production, has been developed.

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