Laparoscopy Endoscopy Cooperative Surgery for Inflammatory Fibroid Polyp in the Esophagus.

Inflammatory fibroid polyp (IFP) appears most often in the stomach. We herein report an extremely rare case of esophageal IFP resected using laparoscopy endoscopy cooperative surgery (LECS). A 73-year-old man with dysphagia underwent esophagogastroduodenoscopy.

[A Case of Laparoscopic-Endoscopic Cooperative Surgery for Duodenal Neuroendocrine Tumor(NET G1)].

We report a case of duodenal neuroendocrine tumor(NET)G1 resected by laparoscopic-endoscopic cooperative surgery (LECS). A 78-year-old woman underwent upper gastrointestinal endoscopy, revealing an 8 mm, rising tumor on the anterior wall of the duodenal bulb. The tumor was pathologically diagnosed as a G1 duodenal NET, by biopsy.

[Quantification of circulating plasma DNA fragments as tumor markers in patients with esophageal and gastric cancer].

The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal and gastric cancer, in order to assess their diagnostic value. Plasma was collected preoperatively from 24 patients with esophageal cancer 53 patients with gastric cancer and from 21 healthy controls. qPCR was performed using two primer sets for the BETA-actin gene, amplifying short (102 bp) and long (253 bp) segments.

Quantification of plasma cell-free DNA in patients with gastric cancer.

Background: The circulating DNA concentration and integrity was examined by a quantitative polymerase chain reaction (qPCR) in the plasma from patients with gastric cancer and their diagnostic value for the detection of gastric cancer assessed.

Patients And Methods: Plasma samples were collected preoperatively from 53 patients with gastric cancer and 21 healthy controls. qPCR was performed using two different primer sets for the beta-actin gene, amplifying short and long segments.

Quantification of circulating plasma DNA fragments as tumor markers in patients with esophageal cancer.

Unlabelled: The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal carcinomas, in order to assess their diagnostic value.

Patients And Methods: Plasma was collected preoperatively from 24 patients with esophageal cancer and 21 healthy controls. qPCR was performed using two primer sets for the beta-actin gene, amplifying short and long segments.

Circulating cell-free mRNA in plasma as a tumor marker for patients with primary and recurrent gastric cancer.

Background: The diagnostic value of circulating mRNA for the early detection of primary and recurrent gastric cancer was evaluated.

Patients And Methods: Circulating hTERT and MUC1 mRNA were amplified in the plasma from 52 gastric cancer patients (40 preoperative and 12 postoperative patients) and 20 healthy controls. The results were compared with those of a circulating cancer cell assay and methylation-specific polymerase chain reaction assay.

Circulating tumor cells and aberrant methylation as tumor markers in patients with esophageal cancer.

Background: This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic approaches on blood samples from patients with esophageal squamous cell cancer.

Materials And Methods: Preoperative blood samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase chain reaction (RT-PCR) assay and methylation-specific polymerase chain reaction (MSP) assay for p16, E-cadherin and RARbeta genes.

Results: Circulating tumor cells were detected in 12 patients (27%); 14 patients (32%) had aberrant methylation in the promoter region of at least one gene (6, 4 and 4 patients, for p16, E-cadherin and RARbeta, respectively).

[An early detection of recurrence using reverse transcriptase-polymerase chain reaction (RT-PCR) and methylation-specific polymerase chain reaction (MSP) from peripheral blood in patients after gastrectomy].

Several molecular approaches, using peripheral blood of patients with cancers, have been assessed recently for ability to detect various primary and recurrent cancers at an early stage. One is the reverse transcriptase polymerase chain reaction (RT-PCR) analysis, which can detect a small number of circulating cancer cells. Another is the methylation-specific polymerase chain reaction (MSP), which detects tumor-specific alterations of cell-free serum DNA released from tumor into the circulation by necrosis and/or apoptosis.

[Plasma methylation-specific polymerase chain reaction as a diagnostic tool for esophageal cancer patients].

This study was designed to perform methylation-specific polymerase chain reaction (MS-PCR) assay for p16, E-cadherin, and retinoic acid receptor beta genes on peripheral blood samples from patients with esophageal squamous cell cancers, and compare the results of MS PCR with conventional serum tumor markers and the CEA-specific reverse transcriptase polymerase chain reaction (RT-PCR) assay. Preoperative blood samples were obtained from 30 patients with esophageal cancer, and were subjected to MS PCR and RT-PCR assays. Eleven patients (37%) showed aberrant methylation of the promoter region of at least one gene.