Oxygenated Scaffolds for Pancreatic Endocrine Differentiation from Induced Pluripotent Stem Cells.

A 3D microenvironment is known to endorse pancreatic islet development from human induced pluripotent stem cells (iPSCs). However, oxygen supply becomes a limiting factor in a scaffold culture. In this study, oxygen-releasing biomaterials are fabricated and an oxygenated scaffold culture platform is developed to offer a better oxygen supply during 3D iPSC pancreatic differentiation.

Angiopoietins stimulate pancreatic islet development from stem cells.

In vitro differentiation of human induced pluripotent stem cells (iPSCs) into functional islets holds immense potential to create an unlimited source of islets for diabetes research and treatment. A continuous challenge in this field is to generate glucose-responsive mature islets. We herein report a previously undiscovered angiopoietin signal for in vitro islet development.

Decellularized Tissue Matrix Enhances Self-Assembly of Islet Organoids from Pluripotent Stem Cell Differentiation.

Regenerating human islet organoids from stem cells remains a significant challenge because of our limited knowledge on cues essential for developing the endocrine organoids in vitro. In this study, we discovered that a natural material prepared from a decellularized rat pancreatic extracellular matrix (dpECM) induces the self-assembly of human islet organoids during induced pluripotent stem cell (iPSC) pancreatic differentiation. For the first time, we demonstrated that the iPSC-derived islet organoids formed in the presence of the dpECM are capable of glucose-responsive secretion of both insulin and glucagon, two major hormones that maintain blood glucose homeostasis.

Exploring the Medicinal Potential of the Fruit Bodies of Oyster Mushroom, Pleurotus ostreatus (Agaricomycetes), against Multidrug-Resistant Bacterial Isolates.

Bacterial resistance to present-generation antibiotics is increasing drastically, which has become a major public health concern. The present study focuses on demonstrating the antimicrobial potential of fruit bodies of the culinary/medicinal oyster mushroom Pleurotus ostreatus against clinical pathogens. Five bacterial isolates were collected from Sagar Hospital in Bangalore, India.

Environmental assessment of the degradation potential of mushroom fruit bodies of Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. towards synthetic azo dyes and contaminating effluents collected from textile industries in Karnataka, India.

Pleurotus ostreatus (Jacq.: Fr.) P.