Changes in faecal bacteria during fattening in finishing swine.

Body fat accumulation in mice and human is linked to the percentage of Firmicutes and Bacteroidetes, two bacterial phyla dominant in the large intestine. However, little is known about the relationship between the composition of the gut microbiota and fattening in pig. This study aimed to investigate the abundance of Firmicutes, Bacteroidetes, and Bacteroides, which is the major genus within Bacteroidetes, in porcine faeces during fattening.

Draft Genome Sequence of the Immunobiotic Strain TL2937.

The genome of the immunomodulatory strain TL2937 is described here. The draft genome has a total length of 1,678,416 bp, a G+C content of 34.3%, and 1,470 predicted protein-coding sequences.

Draft Genome Sequence of Lactobacillus plantarum TL2766, a Strain with the Ability To Ferment Wakame.

The genome sequence of Lactobacillus plantarum TL2766, a strain with the ability to ferment wakame (Undaria pinnatifida), is described here. The reads were assembled into contigs, with a total size of 3,310,195 bp. The genome information will be useful for further specific genetic studies of this strain and for its biotechnological applications.

Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.

Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer's patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L.

Effects of oral administration of Butyrivibrio fibrisolvens MDT-1 on the development and healing of atopic dermatitis in NC/Nga mice.

The purpose of this study was to evaluate the effects of oral administration of Butyrivibrio fibrisolvens MDT-1 (MDT-1) on mite antigen-induced atopic dermatitis (AD) in NC/Nga mice. When administration of the freeze-dried cells of MDT-1 was initiated one week before mite-antigen swabbing, the development of AD-like skin lesions was alleviated. The cell homogenate and membrane fraction had similar effects, showing that cell components are effective.

Dietary sphingomyelin alleviates experimental inflammatory bowel disease in mice.

The effect of dietary sphingomyelin (SM) on inflammatory bowel disease (IBD) induced with dextran sodium sulfate (DSS) was examined in mice. Although the severity of IBD as expressed by the disease activity index (DAI) markedly increased with DSS administration, feeding a diet containing SM lowered the DAI value significantly. Myeloperoxidase (MPO) activity in colonic tissue also increased with DSS administration, suggesting the development of inflammation.

Effect of oral administration of Butyrivibrio fibrisolvens MDT-1, a gastrointestinal bacterium, on 3-methylcholanthrene-induced tumor in mice.

Butyrivibrio fibrisolvens MDT-1 was evaluated for use as a probiotic to prevent tumor formation. Oral administration of MDT-1 (10(9) cfu/dose, 3 times a wk for 15 wk) delayed the onset of tumors induced by 3-methylcholanthrene and also reduced tumor incidence in mice. Furthermore, the numbers of natural killer (NK) and NKT cells in the spleen increased markedly in response to MDT-1 administration.

Effect of oral administration of Butyrivibrio fibrisolvens MDT-1 on experimental enterocolitis in mice.

Butyrivibrio fibrisolvens MDT-1, a butyrate-producing strain, was evaluated for use as a probiotic to prevent enterocolitis. Oral administration of the MDT-1 strain (10(9) CFU/dose) alleviated the symptoms of colitis (including body weight loss, diarrhea, bloody stool, organic disorder, and mucosal damage) that are induced in mice drinking water that contains 3.0% dextran sulfate sodium.

Oral administration of butyrivibrio fibrisolvens, a butyrate-producing bacterium, decreases the formation of aberrant crypt foci in the colon and rectum of mice.

Butyrivibrio fibrisolvens, a butyrate-producing ruminal bacterium, was evaluated for use as a probiotic to prevent colorectal cancer. Oral administration to Jcl:ICR mice of a new strain of B. fibrisolvens (MDT-1) that produces butyrate at a high rate (10(9) cfu/dose) increased the rate of butyrate production by fecal microbes, suggesting that MDT-1 can grow in the gut.

Characterization and transcription of the genes encoding enzymes involved in butyrate production in Butyrivibrio fibrisolvens.

Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not present in this region. The deduced amino acid sequences of these enzymes were similar to those of clostridia.