Method of kinetic characterization of immunoreagents for development of express high-sensitive assays for detection of ochratoxin A and heart fatty acids binding protein.

Development of rapid and sensitive immunoassays is a task of great importance in a variety of fields ranging from clinical practice and urgent diagnostics to food quality control and environmental monitoring. High attention of researches is paid to methods of screening, selection, and kinetic characterization of antibodies that enable fast, specific, and effective formation of immunocomplexes. Herein, we present a method for direct investigation of kinetics of immunoreagents during developments of express high sensitive lateral flow assays.

[Pericarditis in contemporary therapeutic clinic: nosological spectrum, approaches to diagnosis and treatment].

Aim: To analyze the register of pericarditis in a therapeutic clinic, to evaluate their nosological spectrum, to optimize approaches to diagnosis and treatment.

Materials And Methods: For the period 20072018, the register includes 76 patients with the diagnosis of pericarditis (average age 53.115.

Data on characterization of glass biochips and validation of the label-free biosensor for detection of autoantibodies in human serum.

The data represent in-depth characterization of a novel method for highly sensitive simultaneous measuring in human serum of both critical parameters of autoantibodies: concentration and native kinetics. The latter refers to autoantibody interaction with free, not immobilized, antigen. The method and related biosensors are based on the spectral-correlation and spectral-phase interferometry.

Evaluation of occupationally conditioned and non-occupational risks of health disorders in truck drivers.

The authors present analysis of changes in genera! morbidity related to age and length of service for truck drivers (chronic diseases of cardiovascular system, respiratory and digestive organs, urinary system, spine, locomotory system and connective tissue) exposed to complex combination of occupational hazards (unusual work schedule, night shifts, air pollution with automobile transport exhaust gases). Morbid "ity parameters were calculated according to deep medical investigation results in periodic examinations of drivers and their appealability to medical office. General hygienic evaluation of work conditions was performed according to increment in risk of diseases for length of service years (annual risk increase).

[THE AGE AND WORK EXPERIENCE DYNAMICS OF INDICES OF HEALTH OF EMPLOYEES--CRITERIA FOR COMPARISON OF OCCUPATIONAL AND NONOCCUPATIONAL RISKS].

There are reported data of the analysis of parameters of the general morbidity (hypertension, chronic diseases, organs of respiratory and digestive system, adiposity, vertebral osteochondrosis) in dependence on age and work experience of dockers-mechanizators in seaport at various working conditions. Morbidity rate indices were calculated on results of the profound medical examination at annual periodic surveys of workers. There were analyzed ecological and ergonomic indices of working conditions, including an estimation of physiological intensity of the work.

[Evaluating occupational risk in night shift work schedule].

The authors analyzed and summarized risk values of acute and chronic health disorders in night shift workers. Average annual risk increments for various chronic diseases in night shift workers were calculated. Based on these data, the authors conclude on hazard degree of night shift work.

[Physiological and hygienic grounding 8-hour and 12-hour shifts at process production].

On workplaces of an oil refining factory for an estimation of 8-hour and 12-hour shifts the criterion of tiresomeness of work - speed of decrease in working capacity on hours on hours of the working day. Conditions of the industrial environment, physiological intensity of work (density and rate of labour actions), dynamics of parameters of a functional state of an organism of workers, data about symptoms of acute and chronic weariness were analyzed. It is supposed, that at absence of harmful factors of the industrial environment the opportunity of lengthening of a work day till 12-hour is defined by physiological intensity of work - a combination of duration of the working day and week with density and rate of labour actions.

[The stimulation of reparative regeneration in the treatment of fractures of the extremities with the use of new biotechnologies].

The paper presents the results of a study of Russian and foreign authors on modern methods of stimulation of reparative regeneration of bone tissue. Highlight general principles for the construction and use of bioplastic materials. An attempt to classify the most famous of biomaterials based on their physico-chemical and biological properties, as well as identified trends in the further development of tissue engineering and its importance in traumatology and orthopedics.

[Pressing problems of labor hygiene and occupational pathology among office workers].

Northwest public health research center, Ministry of health and social affairs, St.-Petersburg. The article substantiates the conception of "office room", "office worker", estimates the basic diseases and symptoms among office workers (SBS-syndrome, BRI-illnesses, BRS-symptoms).

[Work schedule of electric welders in heating microclimate].

The authors present results of specified hygienically justified timing regulations for setting the variants of work and rest modes within electric welders shift, who work in various postures with variable visual strain while welding in heating microclimate.

[The medical social characteristics of patients hospitalized in the traumatic surgery department].

The medical social characteristics of injured patients hospitalized in the traumatic surgery department of municipal hospital. The study results permitted to develop the organizational functional model of injury prevention management. The pattern presuppose the implementation of complex of activities considering the options of target population groups and measures realization period based on the regular occurrences revealed in the study.

[Investigating occupational risk in visual strain].

The article deals with materials of industrial and laboratory studies on risk of acute and chronic functional overstrain due to visual work in humans. The authors demonstrated value of intensity, structure and duration of visual strain as etiologic risk factors.

[The medical social characteristics of able-bodied population mortality as a result of traffic accidents].

The analysis of the medical social portrait of the deceased in consequence of traffic accidents was implemented. The risk groups and the pattern of mortality indicators distribution according the days of week and seasons were revealed. The major medical social factors of traffic accidents making resulted in fatal outcomes were established considering the characteristics of injuries due to traffic accidents demanding the organization of opportune medical care.

[The management of emergency medical care hospital in force-majeure situation].

The development of the Moscow emergency medical care service depends on the highly social significance of the investigation of mass injuries under force majeure situations and lethality structure. The elaborated and approved algorithm of providing high-quality medical care under mass admission of force-majeure situation victims to the municipal emergency medical care hospital is discussed.

[Using pulse measurements for physiological regulation of work intensity].

The article covers materials of physiologic evaluation of work intensity through pulse measurements. The authors stress drawbacks of present norms for average shift pulse rate. To evaluate work intensity, the researchers suggest normal value of pulse rate change at the 7th work hour vs at the 2nd hour.

[Tumor growth index as an integral criterion for the efficacy of antineoplastic therapy under experimental conditions].

An additional criterion is suggested for description of effectiveness of therapy of experimental tumors--tumor growth index. It is a ratio of area covered by the kinetic curve of tumor growth in the study group and that in control. The index is intended to describe the efficacy of therapy in terms of degree and duration of the effect.

[Regulation of work intensity according to duration, density and pace].

The author describes a method for hygienic regulation of work intensity. The parameters used are "average shift working pace" and "physiologic intensity of work" that summarize three time characteristics of occupational activities--working week duration, density of working time (presence of breaks and pauses within a shift), working pace.

[The integral assessment of subject symptoms for detecting chronic visual, motor and nonspecific fatigue in workers].

The authors present methods of integral evaluation of symptoms for diagnosis of chronic visual, locomotor and nonspecific fatigue in workers. Questionnaires and algorithms to treat them are incorporated into the methods that are to be used in hygienic regulation of work, particularly, to reveal comparative significance of work factors--visual efforts, physical hardiness and nerve strain.

[An anamnestic method for studying the cause-effect connections between the status of public health and manufacturing, ecological and social factors].

The authors elaborated a medical and ecologic questionnaire for specific history. The questionnaire was applied to express-evaluation of public health state and environmental factors in the certain settlement. The health state was proved to be influenced significantly by occupational conditions, hardiness and intensity of work, psychologic climate.

[Quantitative characteristics of the causes of functional overstrain of the hands].

Functional studies of hands strain covered 2351 women who performed multiple manual maneuvers with absent, mild and moderate efforts. Considered reasons of the functional strain were: number of the maneuvers per shift, applied efforts, work intensity, length of service, previous work hardiness, hand efforts beyond the worktime, specific traits of workers (age, health state). Functional strain of hands was assessed by means of an Articular Pain Index which is general evaluation of severity, intensity and frequency of pain in hand joints.

Association of demographic characteristics, knowledge, and worry about HIV infection with occupational and nonoccupational behaviors of medical students.

The authors surveyed a sample of 132 medical students to assess their behaviors related to the risk of HIV infection. Scale measures were computed for survey questions relating to both occupational and nonoccupational behavior and also for questions relating to knowledge of any worry about HIV infection. Regression analysis to assess the influence of demographic characteristics, knowledge, and worry on occupational and nonoccupational behavior indicated that (1) medical students are more likely to implement behaviors to protect themselves from occupational exposure to HIV infection than from nonoccupational exposure and (2) students who worried more about HIV infection reported less at-risk occupational behavior.

Cyclooxygenase products do not modulate angiotensin II-induced contractions of human chorionic plate arteries.

Objective: This study sought to determine whether cyclooxygenase products modulate angiotensin II-induced contractions of human placental arteries.

Study Design: Ring segments of chorionic plate arteries (3 to 5 mm inner diameter) were obtained after normal vaginal deliveries. Active force in response to 10(-6) mol/L angiotensin II or 10(-4) mol/L arachidonic acid was measured in the presence or absence of 10(-6) mol/L indomethacin to inhibit cyclooxygenase.