Enzymatic Synthesis and Structural Confirmation of Novel Oligosaccharide, D-Fructofuranose-linked Chitin Oligosaccharide.

Utilizing transglycosylation reaction catalyzed by β- -acetylhexosaminidase of , β-D-fructofuranosyl-(2↔1)-α- , ´diacetylchitobioside (GlcNAc -Fru) was synthesized from -acetylsucrosamine and , ´-diacetylchitobiose (GlcNAc ), and β-D-fructofuranosyl-(2↔1)-α- , ´, ´´-triacetylchitotrioside (GlcNAc -Fru) was synthesized from GlcNAc -Fru and GlcNAc . Through purification by charcoal column chromatography, pure GlcNAc -Fru and GlcNAc -Fru were obtained in molar yields of 33.0 % and 11.