Publications by authors named "Sophie de Respinis"
Background: The biodiversity of the mycobiota of soft cheese rinds such as Brie or Camembert has been extensively studied, but scant information is available on the fungi colonizing the rinds of cheese produced in the Southern Switzerland Alps. This study aimed at exploring the fungal communities present on rinds of cheese matured in five cellars in Southern Switzerland and to evaluate their composition with regards to temperature, relative humidity, type of cheese, as well as microenvironmental and geographic factors. We used macro- and microscopical morphology, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and sequencing to characterize the fungal communities of the cheeses, and compared them with metabarcoding targeting the ITS region.
View Article and Find Full Text PDFArticle Synopsis
- Several species of Tetraselmis have been found to produce intracellular inclusions of amorphous calcium carbonate, known as micropearls, which are arranged in a pattern specific to each species.
- Early studies noted the presence of these granules, but their mineral nature wasn't understood until now.
- Analysis of chloroplast shapes and molecular genetics has shown correlations between the arrangement of micropearls and the morphology of chloroplasts, confirming the existence of two distinct groups among Tetraselmis species based on these traits.
Article Synopsis
- Protein fingerprinting using MALDI-TOF MS is an effective technique for identifying terrestrial fungi and was evaluated for aquatic hyphomycetes, important fungi in stream ecosystems.
- The study involved 34 isolates from 21 species confirmed through morphology and genetic sequencing, testing various protein extraction methods to optimize mass spectra quality.
- Results showed that MALDI-TOF MS provided reliable and precise identification of aquatic hyphomycetes, outperforming traditional morphological identification methods.
The molecular epidemiology of fish pathogen Saprolegnia parasitica is still largely unknown. We developed a multilocus sequence typing scheme based on seven housekeeping genes to characterize 77 S. parasitica strains isolated from different fish host species at different times and from different geographic areas in Switzerland between 2015 and 2017.
View Article and Find Full Text PDFArticle Synopsis
- - The research aimed to enhance the Vitek MS fungal knowledge base to improve the identification of clinically significant dermatophytes by using diverse strains, incubation times, and growth conditions.
- - A reliable method for sample preparation was developed, leading to accurate identification of 134 strains from 17 species, achieving a high identification performance of 98.4% based on mass spectrum analysis.
- - Validation of the expanded knowledge base with clinical isolates showed successful identification for most taxa, although challenges occurred with certain strains, highlighting the close relation between some species.
In this study we evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of dermatophytes in diagnostic laboratories. First, a spectral database was built with 108 reference strains belonging to 18 species of the anamorphic genera Epidermophyton, Microsporum and Trichophyton. All strains were well characterized by morphological criteria and ITS sequencing (gold standard).
View Article and Find Full Text PDFAnalysis of a worldwide collection of strains of Trichoderma asperellum sensu lato using multilocus genealogies of four genomic regions (tef1, rpb2, act, ITS1, 2 and 5.8s rRNA), sequence polymorphism-derived (SPD) markers, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) of the proteome and classical mycological techniques revealed two morphologically cryptic sister species within T. asperellum, T.
View Article and Find Full Text PDF