A simple method for repeated in vivo sperm collection from laboratory mice.

Purpose: Mouse spermatozoa for archiving laboratory mice or for in vitro fertilization (IVF) are routinely obtained from the cauda epididymis of adult males sacrificed for this purpose. To avoid the death of the donor, we tested whether a precisely timed interruption of the mating act could be used for repeated sperm collection from laboratory mice.

Methods: Sperm donors (B6D2F1) were mated with a receptive female, and mating behavior was observed.