Precise genome editing underlines the distinct contributions of mutations in , , , and genes to antifungal resistance in .

has recently emerged as a major threat due to the worldwide emergence of fluconazole-resistant strains causing clonal outbreaks in hospitals and poses a therapeutic challenge due to the limited antifungal armamentarium. Here, we used precise genome editing using CRISPR-Cas9 to gain further insights into the contribution of mutations in , , , and genes and the influence of allelic dosage to antifungal resistance in . Seven of the most common amino acid substitutions previously reported in fluconazole-resistant clinical isolates (including Y132F in ) were engineered in two fluconazole-susceptible lineages (ATCC 22019 and STZ5).

Automatic patient-level recognition of four species on thin blood smear by a real-time detection transformer (RT-DETR) object detection algorithm: a proof-of-concept and evaluation.

Malaria remains a global health problem, with 247 million cases and 619,000 deaths in 2021. Diagnosis of species is important for administering the appropriate treatment. The gold-standard diagnosis for accurate species identification remains the thin blood smear.

The Novodiag Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study.

Objectives: We provide the first evaluation of the CE-IVD marked Novodiag stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples.

Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP.

Medicopsis romeroi nodular subcutaneous infection in a kidney transplant recipient.

Phaeohyphomycosis is a set of fungal infections caused by various dematiaceous fungi such as coelomycetes. These infections can occur either in immunocompetent or immunocompromised patients like solid organ transplants. Here we describe a nodular lesion of the right hallux that occurred in a kidney transplant patient.