An AIE-based fluorescent dye for selective staining of polyamide microplastics without pretreatment: Applications to environmental samples and zebrafish.

A novel staining dye, BEM ((1E,1'E)-1,1'-([2,2'-bithiophene]-5,5'-diyl)bis(N-(9-ethyl-9H-carbazol-3-yl)methanimine)) was synthesized for selective identification of polyamide (PA) micrplastics. BEM showed unique photophysical properties such as solvatochromism, intramolecular charge transfer (ICT), and aggregation induced emission (AIE) which were demonstrated through spectroscopic analysis and density functional theory (DFT) calculations. The optimal staining conditions for selective staining of PA by BEM were established by evaluating the staining efficiency according to the variation of the solvent compositions, concentrations of BEM, and staining durations.

Multi-bioapplicable fluorescence chemosensor for sequentially identifying gallium ion and pyrophosphate: Application to environmental samples, human serum, zebrafish and celery.

A multi-bioapplicable fluorescence chemosensor MQV, ((E)-2-methoxy-6-((2-(quinolin-2-yl)hydrazineylidene)methyl)phenol), was developed for sequential detection of Ga and pyrophosphate (PPi) ions. The addition of Ga and PPi to MQV in sequence strongly caused an off-on-off fluorescent response in PBS buffer/DMSO (8:2, v/v, pH 7.4).

Fast and easy detection of hypochlorite by a smartphone-based fluorescent turn-on probe: Applications to water samples, zebrafish and plant imaging.

We have developed a fluorescent probe DBT-Cl ((E)-2-(2-(4-(diphenylamino)benzylidene) hydrazinyl)-N,N,N-trimethyl-2-oxoethan-1-aminium chloride) for ClO with an aggregation-induced emission (AIE) strategy depending on solvent polarity. DBT-Cl possessed a prominent solvatochromic emission property with intramolecular charge transfer (ICT) from the TPA (triphenylamine) to the amide group, which was studied by spectroscopic analysis and DFT calculations. These unique AIE properties of DBT-Cl led to the recognition of ClO with high fluorescent selectivity.

Reduced fertility caused by meiotic defects and micronuclei formation during microsporogenesis in xBrassicoraphanus.

Background: Hybridization and polyploidization events are important driving forces in plant evolution. Allopolyploids formed between different species can be naturally or artificially created but often suffer from genetic instability and infertility in successive generations. xBrassicoraphanus is an intergeneric allopolyploid obtained from a cross between Brassica rapa and Raphanus sativus, providing a useful resource for genetic and genomic study in hybrid species.

Meiotic Chromosome Stability and Suppression of Crossover Between Non-homologous Chromosomes in x, an Intergeneric Allotetraploid Derived From a Cross Between and .

Hybridization and polyploidization are major driving forces in plant evolution. Allopolyploids can be occasionally formed from a cross between distantly related species but often suffer from chromosome instability and infertility. x is an intergeneric allotetraploid (AARR; 2n = 38) derived from a cross between (AA; 2n = 20) and (RR; 2n = 18).

Identification and mapping of a novel dominant resistance gene, TuRB07 to Turnip mosaic virus in Brassica rapa.

A novel dominant resistance gene, TuRB07, was found to confer resistance to an isolate of TuMV strain C4 in B. rapa line VC1 and mapped on the top of chromosome A06. The inheritance of resistance to Turnip mosaic virus in Brassica rapa was investigated by crossing the resistant line, VC1 with the susceptible line, SR5, and genotyping and phenotyping diverse progenies derived from this cross.

Construction of a genetic map based on high-throughput SNP genotyping and genetic mapping of a TuMV resistance locus in Brassica rapa.

Brassica rapa is a member of the Brassicaceae family and includes vegetables and oil crops that are cultivated worldwide. The introduction of durable resistance against turnip mosaic virus (TuMV) into agronomically important cultivars has been a significant challenge for genetic and horticultural breeding studies of B. rapa.

Mapping quantitative trait loci for tissue culture response in VCS3M-DH population of Brassica rapa.

Quantitative trait loci (QTL) controlling callus induction and plant regeneration were identified in the VCS3M-DH population of Brassica rapa. Quantitative trait loci (QTL) controlling callus induction and plant regeneration were identified in the VCS3M-DH population of Brassica rapa. The VCS3M-DH population showed wide and continuous variation in callus induction and shoot regeneration.

Developing stable progenies of ×Brassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture.

Induced mutations were used to improve the low seed fertility of an intergeneric allopolyploid, 'Baemoochae,' ×Brassicoraphanus, synthesized following hybridization between Brassica rapa and Raphanus sativus. The mutagen N-methyl-N-nitroso-urethane (NMU) was added to microspore cultures. Four lines of nine in the Mi(2) generation showed very high fertility under controlled pollination.