The landscape of intrinsically disordered proteins in Leishmania parasite: Implications for drug discovery.

Proteins that lack three-dimensional structures are known as Intrinsically disordered proteins (IDPs). In this study, we aimed to identify intrinsically disordered proteins in the Leishmania donovani proteome using various predictors that can identify IDPs based on amino acid residues and charge hydropathy. Top identified IDPs are analyzed using STRING, PSP-Hunter, Deep Loc-2.

A novel approach to design chimeric multi epitope vaccine against exploiting infected host cell proteome.

Leishmaniasis is a major infectious disease having high mortality which could be attributed to lack of a suitable vaccine candidate. We propose a novel approach to design multiepitope vaccine to leishmaniasis exploiting specific membrane proteome from infected macrophage from host. The MHC-I, MHC-II and BC epitopes predicted for unique proteins from the infected macrophages and and a MEV designed in various combinations (1a-1m).

Design and evaluation of a multiepitope vaccine for pancreatic cancer using immune-dominant epitopes derived from the signature proteome in expression datasets.

Pancreatic cancer is a highly aggressive and often lethal malignancy with limited treatment options. Its late-stage diagnosis and resistance to conventional therapies make it a significant challenge in oncology. Immunotherapy, particularly cancer vaccines, has emerged as a promising avenue for treating pancreatic cancer.

Pharmacophore-guided drug design using LdNMT as a model drug target for leishmaniasis.

Leishmaniasis is caused by genus parasites and has a high mortality rate. The available drugs to treat leishmaniasis fail due to acquired resistance in parasites. Several enzymes of the parasite have been used to design new therapeutic molecules against leishmaniasis.

Hemin acts as CD36 ligand to activate down-stream signalling to disturb immune responses and cytokine secretion from macrophages.

Inflammatory responses to hemin are believed to play an important role in tissue damage and cerebral malaria pathology. Macrophage exposed to hemin exhibits modulation of non-opsonic phagocytosis of aged RBCs, ability to kill bacteria and secretion of cytokines. Immuno-fluorescence study indicates translocation and sequestration of CD36 within the intracellular storage in the hemin treated macrophages.

CD36 Ectodomain Detects Apoptosis in Mammalian Cells.

The cells that undergo apoptosis show phosphatidylserine (PS) on the cell membrane. The fluorescently labeled hCD36_ecto is staining and detecting apoptotic cells in a flow-based assay with several advantages over Annexin V. The human CD36 ectodomain (hCD36_ecto) is stable for a range of temperatures and experimental conditions and doesn't require Ca for detecting apoptosis and specific towards PS compared to other lipids.

Therapeutic Potentials of Scavenger Receptor CD36 Mediated Innate Immune Responses Against Infectious and Non-Infectious Diseases.

CD36 is a multifunctional glycoprotein, expressed in different types of cells and known to play a significant role in the pathophysiology of the host. The structural studies revealed that the scavenger receptor consists of short cytosolic domains, two transmembrane domains, and a large ectodomain. The ectodomain serves as a receptor for a diverse number of endogenous and exogenous ligands.

Selective and Sensitive Sensing of Hydrogen Peroxide by a Boronic Acid Functionalized Metal-Organic Framework and Its Application in Live-Cell Imaging.

A new boronic acid functionalized Zr(IV) metal-organic framework having the capability of sensing HO in live cells is reported. The Zr-MOF bears a UiO-66 structure and contains 2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)) as a framework linker. The activated Zr-UiO-66-B(OH) compound (called 1') is highly selective for the fluorogenic detection of HO in HEPES buffer at pH 7.

Mapping of phosphatidylserine recognition region on CD36 ectodomain.

CD36-PS interaction is an important affair to identify and remove dead/aged cells to control inflammation. CD36 ectodomain was cloned, over-expressed in bacterial expression system and purified to homogeneity. The dot-blot analysis shows that the CD36_ecto selectively binds PS vesicles blotted on the nitrocellulose membrane.

A dinitro-functionalized metal-organic framework featuring visual and fluorogenic sensing of HS in living cells, human blood plasma and environmental samples.

Here, we describe a new dinitro-functionalized Zr(iv) MOF (MOF = metal-organic framework) having a UiO-66 (UiO = University of Oslo) framework topology called UiO-66-(NO) (1). It shows fluorescence turn-on behavior towards HS in simulated biological medium (HEPES buffer, pH = 7.4).

Extraordinary sensitivity for HS and Fe(iii) sensing in aqueous medium by Al-MIL-53-N metal-organic framework: in vitro and in vivo applications of HS sensing.

An Al(iii) metal-organic framework (MOF) called Al-MIL-53-N (1) was synthesized under solvothermal reaction conditions using Al(NO)·9HO and HBDC-N (HBDC-N = 2-azido-1,4-benzenedicarboxylic acid) ligand in a DMF/water (DMF = N,N-dimethylformamide) mixture. Phase purity was checked by performing X-ray powder diffraction, infrared spectroscopy and thermogravimetric analysis. Thermogravimetric analysis suggests that 1 is highly stable up to 300 °C under air atmosphere.

Rapid and highly sensitive detection of extracellular and intracellular HS by an azide-functionalized Al(iii)-based metal-organic framework.

A new, azide-functionalized Al(iii)-based metal-organic framework (MOF) denoted as CAU-10-N (1, CAU = Christian-Albrechts-University) and consisting of the 5-azido-isophthalic acid (HIPA-N) ligand was employed as a reaction-based fluorescent turn-on probe for the detection of HS. The activated compound (1') showed fast, selective and highly sensitive sensing properties for extracellular HS in HEPES buffer (10 mM, pH = 7.4).

Chemoenzymatic Route for the Synthesis of (S)-Moprolol, a Potential β-Blocker.

A biocatalytic route for the synthesis of a potential β-blocker, (S)-moprolol is reported here. Enantiopure synthesis of moprolol is mainly dependent on the chiral intermediate, 3-(2-methoxyphenoxy)-propane-1,2-diol. Various commercial lipases were screened for the enantioselective resolution of (RS)-3-(2-methoxyphenoxy)propane-1,2-diol to produce the desired enantiomer.