Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode.

Recently, bacterial endophytes (BEs) have gained importance in the agricultural sector for their use as biocontrol agents to manage plant pathogens. Outbreak of the pine wilt disease (PWD) in Korea has led researchers to test the feasibility of BEs in controlling the pine wood nematode (PWN) Bursaphelenchus xylophilus. In this study, we have reported the diversity and biocontrol activity of BEs against the PWN.

Genomic Insights into Nematicidal Activity of a Bacterial Endophyte, MG against Pine Wilt Nematode.

Pine wilt disease, caused by the nematode , is one of the most devastating conifer diseases decimating several species of pine trees on a global scale. Here, we report the draft genome of MG, which is isolated from mountain-cultivated ginseng plant as an bacterial endophyte and shows nematicidal activity against . Our analysis of MG genome showed that it possesses many genes encoding potential nematicidal factors in addition to some secondary metabolite biosynthetic gene clusters that may contribute to the observed nematicidal activity of the strain.

Publisher Correction: Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea.

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Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea.

Plants harbor diverse communities of bacterial species in their internal compartments. Here we isolated and identified bacterial endophytes from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) to make working collection of endophytes and exploit their potentially beneficial properties toward plants and human being. A total of 1,886 bacteria were isolated from root, stem and leaf of MCGs grown in 24 different sites across the nation, using culture-dependent approach.

Characterization of gibberellin biosynthetic gene cluster from Fusarium proliferatum.

Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center for Biotechnology Information (NCBI) under accession numbers EF119831, EF119832, DQ313173, DQ313174, DQ313175, DQ313176, and DQ313177.

Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.

A novel lipolytic enzyme was isolated from a metagenomic library after demonstration of lipolytic activity on an LB agar plate containing 1% (w/v) tributyrin. A novel esterase gene (estIM1), encoding a lipolytic enzyme (EstIM1), was cloned using a shotgun method from a pFosEstIM1 clone of the metagenomic library, and the enzyme was characterized. The estIM1 gene had an open reading frame (ORF) of 936 base pairs and encoded a protein of 311 amino acids with a molecular mass 34 kDa and a pI value of 4.