Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.

Cottonseed meal (CSM) is a good source of dietary proteins but is unsuitable for human consumption due to its gossypol content. To unlock its potential, we developed a protein extraction process with a gossypol removal treatment to generate CSM protein isolate (CSMPI) with ultra-low gossypol content. This process successfully reduced the free and total gossypol content to 4.

Clinical Study on Plant Galactagogue Worldwide in Promoting Women's Lactation: a Scoping Review.

Human milk is recommended by the World Health Organization (WHO) for the general well-being of infants. However, many mothers face an insufficient milk supply to breastfeed their children. Galactagogue, in particular, plant galactagogue, serves as a method to promote lactation.

Isolation and identification of O- and N-linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth.

Milk serves as the sole nutrition for newborns, as well as a medium for the transfer of immunological components from the mother to the baby. This study reveals different glycoprotein profiles obtained from human, bovine, and caprine milk and their potential roles in supporting infant growth. Proteins from these three milk samples are separated and analyzed using two-dimensional gel electrophoresis (2-DE).

Development of a rapid and improved two-dimensional electrophoresis method for the separation of protein lysate.

Researchers frequently use two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) prior to mass spectrometric analysis in a proteomics approach. The i2D-PAGE method, which 'inverts' the dimension of protein separation of the conventional 2D-PAGE, is presented in this publication. Protein lysate of Channa striata, a freshwater snakehead fish, was separated based on its molecular weight in the first dimension and its isoelectric point in the second dimension.

Bioactive Proteins in Channa striata Promote Wound Healing through Angiogenesis and Cell Proliferation.

Background: Channa striata are speculated to contain bioactive proteins with the ability to enhancing wound healing. It is commonly consumed after surgery for a faster recovery of the wound.

Objective: To identify the bioactive proteins and evaluate their ability in cell proliferation and angiogenesis promotion.

Proteome analysis of excretory-secretory proteins of HM1:IMSS via LC-ESI-MS/MS and LC-MALDI-TOF/TOF.

Background: Excretory-secretory (ES) proteins of are thought to play important roles in the host invasion, metabolism, and defence. Elucidation of the types and functions of ES proteins can further our understanding of the disease pathogenesis. Thus, the aim of this study is to use proteomics approach to better understand the complex ES proteins of the protozoa.