Objective: We describe a technique that records the sensory nerve action potential (SNAP) of the medial plantar proper digital nerve (MPPDN), which is the final sensory branch of the medial plantar nerve.
Methods: We obtained antidromic MPPDN responses from 118 healthy volunteers, with surface recording electrodes placed on the medial side of the first metatarsal head of the great toe, with electrical stimulation applied at the 40% distance of the heel to great toe reference line. Antidromic SNAP parameters were recorded and normative reference values were obtained.
The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, transgene containment, and a lack of gene silencing and position effects. The extension of plastid transformation technology to monocotyledonous cereal crops, including rice, bears great promise for the improvement of agronomic traits, and the efficient production of pharmaceutical or nutritional enhancement. Here, we report a promising step towards stable plastid transformation in rice.
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