Cholinergic Nerve Differentiation of Mesenchymal Stem Cells Derived from Long-Term Cryopreserved Human Dental Pulp In Vitro and Analysis of Their Motor Nerve Regeneration Potential In Vivo.

The reduction of choline acetyltransferase, caused by the loss of cholinergic neurons, leads to the absence of acetylcholine (Ach), which is related to motor nerve degeneration. The aims of the present study were to evaluate the in vitro cholinergic nerve differentiation potential of mesenchymal stem cells from cryopreserved human dental pulp (hDPSCs-cryo) and to analyze the scale of in vivo motor nerve regeneration. The hDPSCs-cryo were isolated and cultured from cryopreserved dental pulp tissues, and thereafter differentiated into cholinergic neurons using tricyclodecane-9-yl-xanthogenate (D609).

Web-based comprehensive information system for self-management of diabetes mellitus.

Background: The aim of this study is to evaluate the effect of a web-based comprehensive information system, consisting of Internet and cellular phone use, on blood glucose control.

Methods: We established eMOD (electronic Management of Diabetes), a web-based ubiquitous information system, for cell phone users along with a website for Internet users to provide diabetes education. We examined whether this information system has the same impact on glycemic control as conventional education for the diabetes patient.