Publications by authors named "Sookie La"
Article Synopsis
- - The study investigates the effects of microneedle patches attached to specific acupuncture points on improving Parkinson’s disease (PD) symptoms and restoring immune function, as immune dysfunction is a major contributor to neuroinflammation in PD.
- - In a PD mouse model, microneedle patches showed significant improvement in motor skills, reduced neuronal death, and lowered neuroinflammation compared to sham patches over a 14-day period.
- - The research indicates that the microneedle patches help restore the disrupted immune response by correcting the CD4+/CD8+ ratio and reducing harmful cytokine levels in the brain and blood, providing a potential new treatment approach for PD.
Purpose: A new fixed-dose combination (FDC) formulation of telmisartan 80 mg and S-amlodipine 5 mg (CKD-828) has been developed to increase convenience (as only one tablet is required per day) and improve treatment compliance.
Methods: The pharmacokinetic characteristics and tolerability of an FDC of telmisartan and S-amlodipine were compared to those after coadministration of the individual agents in this randomized, open-label, single-dose, two-way, four-period, crossover study. To analyze the telmisartan and S-amlodipine plasma concentrations using a validated liquid chromatography-tandem mass spectrometry method, serial blood samples were collected up to 48 hours post-dose for telmisartan and 144 hours post-dose for S-amlodipine, in each period.
A liquid chromatography-triple quadrupole mass spectrometric (LC-MS/MS) method was developed and validated for the determination of 5-nitro-5'-hydroxy-indirubin-3'-oxime (AGM-130) in human plasma to support a microdose clinical trial. The method consisted of a liquid-liquid extraction for sample preparation and LC-MS/MS analysis in the positive ion mode using TurboIonSpray(TM) for analysis. d3 -AGM-130 was used as the internal standard.
View Article and Find Full Text PDFMultivariate pattern recognition (PR) analysis combined with LC/MS was utilized to evaluate the feasibility of predicting chemical-induced toxicity in rats. Urine samples were collected from rats treated with vehicles or four hepatotoxins, alpha-naphthyl isothiocyanate (ANIT), carbontetrachloride (CCl4), acetaminophen, and diclofenac, and analyzed by HPLC coupled with electrospray mass spectrometry. Chromatographic data were normalized using modified Z score transformation with those of the control group, to remove the vehicle effects for a further enhanced multivariate analysis.
View Article and Find Full Text PDFA rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of oxiracetam in human plasma. Oxiracetam, a highly polar compound, was successfully retained by Atlantis dC18 reversed-phase column and detected with triple-quadrupole tandem mass spectrometry. After addition of internal standard (piracetam) to human plasma, plasma was simply precipitated with two volume of acetonitrile, evaporated and dissolved in 0.
View Article and Find Full Text PDFA rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of oxiracetam in human plasma. After addition of internal standard (piracetam) plasma was precipitated with two volumes of acetonitrile and the supernatant was evaporated. The residues were dissolved in 0.
View Article and Find Full Text PDFThe metabolism of a novel anti-cancer agent, 1-(3-[3-(4-cyanobenzyl)-3H-imidazol-4-yl]-propyl)-3-(6-methoxypyridin-3-yl)-1-(2-trifluoromethylbenzyl)thiourea (YH3945), was investigated in rats. Bile, plasma, feces, and urine were collected and analyzed by a high-performance liquid chromatography (HPLC) system equipped with ultraviolet (UV), mass spectrometric, and radioactivity detectors. After intravenous dosing, mean radiocarbon recovery was 74.
View Article and Find Full Text PDFSimultaneous enantioseparations of nine profens for their accurate chiral discrimination were achieved by capillary electrophoresis (CE) in the normal polarity (NP) mode with a single cyclodextrin (CD) system and in the reversed polarity (RP) mode with a dual CD system. The single CD system in the NP mode employed heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin (TMbetaCD) added at 75 mM-100 mM 2-(N-morpholino)ethanesulfonic acid buffer (pH 6.0) as the optimum run buffer.
View Article and Find Full Text PDFArticle Synopsis
- Researchers accomplished simultaneous separation of 15 racemic aromatic amino acids and L-mimosine through two modified capillary electrophoresis (CE) techniques using different cyclodextrins (CDs) as selectors.
- Hydroxypropyl (HP)-alpha-CD provided good chiral environments for most amino acids, while highly sulfated (HS)-gamma-CD achieved high-resolution separations for nearly all enantiomers except for L-mimosine.
- The methods showed high precision and adequate linearity for chiral assays, enabling detection of nonprotein amino acids from plant seeds, such as L-mimosine and L-3,4-dihydroxyphenylalanine.
Article Synopsis
- A new capillary electrophoresis (CE) system was developed that combines two techniques, capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC), to analyze 23 nonprotein amino acids and 4 protein amino acids.
- The system uses uncoated silica capillary methods under specific buffer conditions for each technique, resulting in different migration patterns for the amino acids.
- The method shows good repeatability and detection limits, successfully identifying amino acids in plant seeds, including mimosine and 2-phenylglycine, alongside known aromatic amino acids like tryptophan and phenylalanine.