The objective of this study was to evaluate the effect of chitosan microparticles on the uterine microbiome of cows with metritis. Dairy cows with metritis ( = 89) were assigned to 1 of 3 treatments: chitosan microparticles ( = 21), in which the cows received an intrauterine infusion of chitosan microparticles at metritis diagnosis (day 0), day 2, and day 4; ceftiofur ( = 25), in which the cows received a subcutaneous injection of ceftiofur on day 0 and day 3; and no intrauterine or subcutaneous treatment ( = 23). Nonmetritic cows ( = 20) were healthy cows matched with cows with metritis by the number of days postpartum at metritis diagnosis.
View Article and Find Full Text PDFWe report here the complete genome sequence ofEscherichia coliO157:H7 strain JEONG-1266 isolated from a super- shedder steer in northwest Florida. Cattle are considered a primary reservoir ofE. coliO157:H7, and those cattle that excrete this pathogen in their feces at levels ≥10(4) CFU/g are known as super-shedders.
View Article and Find Full Text PDFProstate cancer is the second leading cause of cancer death in men worldwide. In the present study, we examined in vitro and in vivo antitumor effect of the small molecule imiquimod, also known as a TLR7 agonist, against prostate cancer. Imiquimod inhibited the growth of mouse (TRAMP‑C2) and human (PC-3) prostate cancer cells.
View Article and Find Full Text PDFTLR4 is a membrane sensor for lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria. In this study, we investigated the role of TLR4 on innate immune responses in immune cells against Acinetobacter baumannii. Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were isolated from WT and TLR4-deficient mice and infected with A.
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