Analyzing the accuracy of a cross-mounting technique utilizing digitized interocclusal records.

Purpose: To compare the accuracy of a partially digital cross-mounting workflow of direct scans of interocclusal records to a conventional workflow by analyzing the deviations of sequentially cross-mounted casts.

Materials And Methods: A set of reference casts, comprising maxillary and mandibular full-arch prepared casts and interim prostheses, was articulated, mounted, and scanned to generate four reference casts for cross-mounting. In the conventional approach, 15 sets of these four casts were printed.

Comparison of accuracy in digital and conventional cross-mounting.

Statement Of Problem: The use of digital interocclusal registration scans for virtual articulation and mounting has been studied extensively; however, the accuracy of the cross-mounting procedures in a digital workflow is not well understood.

Purpose: The purpose of this in vitro study was to compare the accuracy of digital and conventional cross-mounting by measuring the 3-dimensional deviation at each step of sequential cross-mounting.

Material And Methods: A set of reference casts and complete arch interim restorations was prepared for complete arch complete-coverage restorations, hand-articulated, and mounted in an articulator.

Regenerative Treatment of Advanced Peri-implantitis: A Retrospective Case Series with 3 to 15 Years of Follow-up.

The present case series evaluated implant survival and changes in bleeding on probing (BOP), probing depths, marginal tissue levels (recession), and bone levels following regenerative treatment of implants with advanced peri-implantitis. Forty-six implants with advanced peri-implantitis in 38 patients were treated with a regenerative protocol and evaluated for the above parameters at the final follow-up 3 to 15 years later. Patients returned every 2 to 3 months for maintenance and at the final evaluation.

A Retrospective Study of Bone-Level Stability Around 335 Hybrid Implants Placed with an Immediate or Delayed Protocol.

This retrospective study evaluated 335 hybrid implants placed in edentulous ridges; 167 were placed with an immediate implant protocol (IIP) and 168 were placed with a delayed implant protocol. Radiographic bone level changes were measured 1 to 23 years after loading. The average bone loss between implant placement and second-stage loading was 0.

Comparison of Intraoral and Extraoral Digital Scanners: Evaluation of Surface Topography and Precision.

The aim of this study was to evaluate the surface topography and the precision measurements of different intraoral and extraoral digital scanners. A reference model of a maxillary arch with four implant analogs was prepared and scanned by three intraoral and two extraoral scanners. The reference model was scanned fifteen times with each digital scanning system, investigating the surface topography and precision measurements for the same-arch and cross-arch measurements.

Physical impacts of PLGA scaffolding on hMSCs: Recovery neurobiology insight for implant design to treat spinal cord injury.

Our earlier work generated a powerful platform technology of polymeric scaffolding of stem cells to investigate and treat the injured or diseased central nervous system. However, the reciprocal sequelae between biophysical properties of the polymer and responses of the stem cell have not been examined in situ in lesioned spinal cords. We postulated that implantable synthetic scaffolds, acting through physical features, might affect donor cell behavior and host tissue remodeling.

Prospective 1-Year Study of Immediately Loaded Implants: 8-Patient Case Series Report.

The goal of this case series report is to demonstrate the predictability of providing immediate restorations with an implant specifically designed to achieve high primary stability, and to report on 12-month survival outcomes. Twenty-nine implants were placed in eight patients with various tooth-replacement needs, from single teeth to partially edentulous spans to fully edentulous mandibles. A new tapered-implant system (The Marc Nevins, Little Implant) was utilized that incorporates a self-tapping thread design with a thread pitch and thread lead to improve primary stability at insertion.

Effectiveness of a Flipped Classroom in Learning Periodontal Diagnosis and Treatment Planning.

The aim of this study was to assess whether a flipped classroom was an effective model for dental students to learn periodontal diagnosis and treatment planning (DTP). Participants were all third-year students in three academic years (2015-17) at Harvard School of Dental Medicine: two groups that experienced the flipped classroom (Classes of 2017 and 2018), and a control group (Class of 2019) that received the same content in traditional lecture format. All three groups completed a DTP knowledge quiz before and after the educational experience; the flipped classroom groups also completed pre and post surveys of their opinions about flipped classrooms.

Defining recovery neurobiology of injured spinal cord by synthetic matrix-assisted hMSC implantation.

Mesenchymal stromal stem cells (MSCs) isolated from adult tissues offer tangible potential for regenerative medicine, given their feasibility for autologous transplantation. MSC research shows encouraging results in experimental stroke, amyotrophic lateral sclerosis, and neurotrauma models. However, further translational progress has been hampered by poor MSC graft survival, jeopardizing cellular and molecular bases for neural repair in vivo.

Using the Theory of Planned Behavior to Identify Predictors of Oral Hygiene: A Collection of Unique Behaviors.

Background: This study aims to identify predictors of performed oral hygiene behaviors (OHBs) based on the Theory of Planned Behavior (TPB), oral health knowledge, and demographic factors.

Methods: Using a questionnaire, 381 participants in three general dental offices and one hospital dental department in York, Pennsylvania, were surveyed regarding performed OHB, attitudes, subjective norms, perceived behavioral control, oral health knowledge, income, age, and sex.

Results: Three unique elements of OHB were identified for analysis: brushing, interdental cleaning, and tongue cleaning.

A 10-year retrospective radiographic study of implantium dental implants.

A retrospective radiographic study was conducted from five private dental offices on patients requesting dental implant therapy to replace their missing teeth. All implants were placed and restored by early 2000, and patients were encouraged to continue their dental care at the same office. The records were reviewed and analyzed by the clinicians, who had more than 15 years of dental experience at the time of the initial patient treatment.

Stemness enhancement of human neural stem cells following bone marrow MSC coculture.

Rapid loss of stemness capacity in purified prototype neural stem cells (NSCs) remains a serious challenge to basic and clinical studies aiming to repair the central nervous system. Based on the essential role of mesodermal guidance in the process of neurulation, we hypothesized that coculture of human NSCs (hNSCs) with human bone marrow-derived mesenchymal stromal stem cells (hMSCs) could enhance the stemness of hNSCs through Notch-1 signaling. We have now tested the hypothesis by assessing behaviors of hNSCs and hMSCs under systematically designed coculture conditions relative to monocultures, with or without Notch-1 manipulation in vitro.

Efficacy of growth factor in promoting early osseointegration.

A preclinical study was conducted to evaluate the feasibility of 2 different topical formulations of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) to promote early osseointegration and enhanced bone-to-implant contact (BIC) for dental implants placed in an edentulous ridge. Six female beagle dogs were divided into 3 groups. The control group included 4 implants with no coating; test group A included 10 implants with commercially available rhPDGF-BB formulation coating; and second test group B included 10 implants with prototype viscous rhPDGF-BB coating.

A prospective 9-month human clinical evaluation of Laser-Assisted New Attachment Procedure (LANAP) therapy.

This investigation was designed and implemented as a single-center, prospective study to evaluate the clinical response to the Laser-Assisted New Attachment Procedure (LANAP). Eight patients with advanced periodontitis were enrolled and treated with full-mouth LANAP therapy and monitored for 9 months. Fullmouth clinical measurements, including clinical attachment level (CAL), probing depth (PD), and recession, were provided at baseline and after 9 months of healing by a single calibrated examiner, including a total of 930 sites and 444 sites with initial PD equal to or greater than 5 mm.

The clinical and histologic outcome of dental implant in large ridge defect regenerated with alloplast: a randomized controlled preclinical trial.

A basic tenet of successful osseointegration is that the implant resides in a sufficient quality and quantity of bone to ensure bone contact and thus stabilization. A prospective, randomized controlled preclinical trial was conducted to evaluate the bone-to-implant contact (BIC) when placing implants in bone regenerated by 3 different combinations of biphasic calcium phosphate (BCP). Dental implants were placed into the regenerated ridges of 6 female foxhounds; the ridges were reconstructed with different formulations of BCP in combination with an hydroxyapatite collagen membrane.

A prospective, randomized controlled preclinical trial to evaluate different formulations of biphasic calcium phosphate in combination with a hydroxyapatite collagen membrane to reconstruct deficient alveolar ridges.

Many patients and clinicians would prefer a synthetic particulate bone replacement graft, but most available alloplastic biomaterials have limited osteogenic potential. An alloplast with increased regenerative capacity would be advantageous for the treatment of localized alveolar ridge defects. This prospective, randomized controlled preclinical trial utilized 6 female foxhounds to analyze the osteogenic impact of different formulations of biphasic calcium phosphate (BCP) in combination with an hydroxyapatite-collagen membrane and their ability to reconstruct deficient alveolar ridges for future implant placement.

Human clinical and histologic evaluation of laser-assisted new attachment procedure.

This investigation was designed to evaluate the healing response to the laser-assisted new attachment procedure (LANAP). Eight patients presenting with 12 teeth predetermined to be surgically extracted were enrolled and consented to treatment with full-mouth LANAP therapy. LANAP surgical therapy consisted of a first pass with a 360-Μm fiber diameter, laser settings with verified output of 4.

The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression.

Purpose: The aim of this study was to investigate the cellular response of human gingival fibroblasts (HGFs) cultured on smooth or rough zirconia (Zr) or titanium (Ti) disks.

Materials And Methods: Disks fabricated from four different materials--smooth Zr (Zr-S), rough Zr (Zr-R), smooth Ti (Ti-S), and rough Ti (Ti-R)--were used, and surface roughness was analyzed by atomic force microscopy. After HGFs were cultured on these disks, cell morphology was examined by scanning electron microscopy, cell proliferation activity was evaluated by a monotetrazolium assay, and gene expression levels of various collagens and integrins were measured by real-time polymerase chain reaction.

The combination of purified recombinant human platelet-derived growth factor-BB and equine particulate bone graft for periodontal regeneration.

Background: The objective of this study is to evaluate the potential for periodontal regeneration of a critical-sized defect with the application of recombinant human platelet-derived growth factor (rhPDGF-BB) combined with either a particulate equine or a β-tricalcium phosphate (β-TCP) matrix.

Methods: Critical-sized intrabony 2-wall defects were created bilaterally on the distal surface of the second premolar and the mesial surface of the first molar in nine hounds. Twelve defects received rhPDGF-BB/equine treatment, 12 defects received rhPDGF-BB/β-TCP treatment, and the remaining 12 sites served as sham-surgery controls.

Human buccal plate extraction socket regeneration with recombinant human platelet-derived growth factor BB or enamel matrix derivative.

The objective of this study was to assess the osseous healing of buccal plate extraction socket defects. There were four cohorts: group A (mineral collagen bone substitute [MCBS] scaffold alone), group B (MCBS with recombinant human platelet-derived growth factor BB [rhPDGF-BB; 0.3 mg/mL]), group C (MCBS with enamel matrix derivative [EMD]), and group D (combination of EMD with bone ceramic).

The use of mucograft collagen matrix to augment the zone of keratinized tissue around teeth: a pilot study.

This prospective split-mouth pilot case series compared the use of a bilayer collagen matrix (CM) to an autogenous gingival graft (AGG) in the ability to increase the zone of keratinized attached gingiva. Five patients with inadequate amounts of keratinized attached gingiva bilaterally in the posterior mandible were enrolled using a split-mouth design. There were statistically significant increases in attached gingiva at all test (CM) and control (AGG) sites.

Factors associated with dental implant survival: a 4-year retrospective analysis.

Background: Dental implants are a predictable treatment option for replacing missing teeth and have strong survival and success outcomes. However, previous research showed a wide array of potential risk factors that may have contributed to dental implant failures. The objectives of this study are to study if implant survival rates were affected by known risk factors and risk indicators that may have contributed to implant failures.

The feasibility of demineralized bone matrix and cancellous bone chips in conjunction with an extracellular matrix membrane for alveolar ridge preservation: a case series.

An investigation was conducted to test the feasibility of demineralized bone matrix and cancellous bone chips in a reverse-phase medium carrier (DynaBlast) in concert with an extracellular matrix membrane (DynaMatrix) to provide hard and soft tissue regeneration for the purpose of a ridge preservation procedure. Nine patients requiring extraction of 30 maxillary teeth were grafted with DynaBlast and DynaMatrix. Twenty sites attained primary flap closure over the grafted area (primary healing intention group), while 10 sites were assigned randomly to the secondary healing intention group, in which primary flap closure over the membrane was not achieved.

The acrosomal vesicle of mouse sperm is a calcium store.

Subsequent to binding to the zona pellucida, mammalian sperm undergo a regulated sequence of events that ultimately lead to acrosomal exocytosis. Like most regulated exocytotic processes, a rise in intracellular calcium is sufficient to trigger this event although the precise mechanism of how this is achieved is still unclear. Numerous studies on mouse sperm have indicated that a voltage-operated Ca2+ channel plays some immediate role following sperm binding to the zona pellucida glycoprotein ZP3.