Publications by authors named "Sonia Prado-Lopez"
With cancer as one of the leading causes of death worldwide, there is a need for the development of accurate, cost-effective, easy-to-use, and fast drug-testing assays. While the NCI 60 cell-line screening as the gold standard is based on a colorimetric assay, monitoring cells electrically constitutes a label-free and non-invasive tool to assess the cytotoxic effects of a chemotherapeutic treatment on cancer cells. For decades, impedance-based cellular assays extensively investigated various cell characteristics affected by drug treatment but lack spatiotemporal resolution.
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- SIEVE is a new statistical method designed to analyze genetic data from single-cell DNA sequencing, focusing on identifying somatic variants and understanding cell lineage.* -
- It improves on existing techniques by using raw read counts and correcting biases in estimating branch lengths, showing better performance in both phylogenetic reconstruction and variant calling, especially for homozygous variants.* -
- When applied to datasets for triple-negative breast cancer and colorectal cancer, SIEVE revealed that double mutant genotypes are uncommon in colorectal cancer but surprisingly common in triple-negative breast cancer samples.*
Article Synopsis
- The study investigates how analyzing circulating tumor cells (CTCs) from blood can provide valuable insights into cancer treatment effectiveness and survival predictions for metastatic colorectal cancer (mCRC) patients.
- Researchers compared different methods for collecting CTCs—CellSearch, Parsortix, and FACS—and found that the genomic mutations varied based on the method used.
- Results showed that CTCs from Parsortix and CellSearch methods presented genomic features similar to those of the primary tumors, while FACS enrichment resulted in misleading diversity estimates due to sequencing errors; this underscores the potential of CTC analysis in personalizing cancer treatment.
Article Synopsis
- - The recurrence of tumor cells in metastatic colorectal cancer (mCRC) poses a major challenge even after surgical removal of metastases, highlighting the need to study the evolution of these recurrent tumors.
- - A unique dataset from single-cell whole-genome sequencing of recurrent liver lesions in an mCRC patient revealed that a certain tumor lineage survived surgery and continued to evolve, despite undergoing chemotherapy for two years.
- - The study found that chemotherapy significantly contributed to mutations in tumor cells, suggesting that some mCRC subclones can migrate and adapt after treatment, leading to rapid regrowth of tumors.
Article Synopsis
- Human mitochondria can be genetically different within the same person, a situation called heteroplasmy, particularly prevalent in cancer.
- In a study of colorectal cancer patients, researchers sequenced individual mitochondrial genomes from normal and tumor cells and found that both types of cells carry diverse mitochondrial genetic variations.
- The study indicates that this variation can exist before cancer starts and persist in certain tumor cell groups, but the somatic mutations observed in these cells don't seem to significantly contribute to cancer development.
How and when tumoral clones start spreading to surrounding and distant tissues is currently unclear. Here we leveraged a model-based evolutionary framework to investigate the demographic and biogeographic history of a colorectal cancer. Our analyses strongly support an early monoclonal metastatic colonization, followed by a rapid population expansion at both primary and secondary sites.
View Article and Find Full Text PDFCorrigendum to "Macroalgae to nanoparticles: Study of Ulva lactuca L. role in biosynthesis of gold and silver nanoparticles and of their cytotoxicity on colon cancer cell lines" [Mater. Sci. Eng. C 97 (2019) 498-509].
Mater Sci Eng C Mater Biol Appl
August 2019
Macroalgae to nanoparticles: Study of Ulva lactuca L. role in biosynthesis of gold and silver nanoparticles and of their cytotoxicity on colon cancer cell lines.
Mater Sci Eng C Mater Biol Appl
April 2019
Marine bio-resources are being widely studied as an invaluable source of compounds with therapeutic applicability. In particular, macroalgae contain an extended variety of bioactive compounds with different structures and promising biological applications. In this work, Ulva lactuca L.
View Article and Find Full Text PDFMultipotent mesenchymal stromal cells are multipotent cells capable of differentiating into different mesodermal cell types. Enigmatically, mesenchymal stromal cells present in the bone marrow support early lymphopoiesis yet can inhibit mature lymphocyte growth. Critical features of the bone marrow microenvironment, such as the level of oxygen, play an important role in mesenchymal stromal cell biology.
View Article and Find Full Text PDFEarly development of mammalian embryos occurs in an environment of relative hypoxia. Nevertheless, human embryonic stem cells (hESC), which are derived from the inner cell mass of blastocyst, are routinely cultured under the same atmospheric conditions (21% O(2)) as somatic cells. We hypothesized that O(2) levels modulate gene expression and differentiation potential of hESC, and thus, we performed gene profiling of hESC maintained under normoxic or hypoxic (1% or 5% O(2)) conditions.
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