The effects of addition of divalent cations and phosphate buffer on the degree of beta-carotene encapsulation in a mannitol matrix during freeze-drying were analyzed. The degradation rate of encapsulated beta-carotene as a function of % RH and its relationship with the physical state of the matrix during storage at 25 degrees C was also studied. The presence of phosphate salts significantly delayed mannitol crystallization at a highly satisfactory degree during freeze-drying and, consequently, the degree of beta-carotene encapsulation increased.
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